2o9l: Difference between revisions
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New page: left|200px<br /><applet load="2o9l" size="350" color="white" frame="true" align="right" spinBox="true" caption="2o9l" /> '''AMBER refined NMR Structure of the Sigma-54 ... |
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== | ==AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element== | ||
The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene | <StructureSection load='2o9l' size='340' side='right'caption='[[2o9l]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2o9l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O9L FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o9l OCA], [https://pdbe.org/2o9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o9l RCSB], [https://www.ebi.ac.uk/pdbsum/2o9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o9l ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O66858_AQUAE O66858_AQUAE] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o9/2o9l_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o9l ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP. | |||
Structural basis of DNA recognition by the alternative sigma-factor, sigma54.,Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658<ref>PMID:17481658</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2o9l" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Sigma factor 3D structures|Sigma factor 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Doucleff | [[Category: Doucleff M]] | ||
[[Category: Lee | [[Category: Lee PS]] | ||
[[Category: Pelton | [[Category: Pelton JG]] | ||
[[Category: Wemmer | [[Category: Wemmer DE]] | ||
Latest revision as of 00:16, 28 December 2023
AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element
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