3mbz: Difference between revisions

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[[Image:3mbz.jpg|left|200px]]


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==OXA-24 beta-lactamase complex soaked with 10mM SA4-17 inhibitor for 15min==
The line below this paragraph, containing "STRUCTURE_3mbz", creates the "Structure Box" on the page.
<StructureSection load='3mbz' size='340' side='right'caption='[[3mbz]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3mbz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MBZ FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MXC:(2S,3R)-2-[(7-AMINOCARBONYL-2-METHANOYL-INDOLIZIN-3-YL)AMINO]-4-AMINOCARBONYLOXY-3-METHYL-3-SULFINO-BUTANOIC+ACID'>MXC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3mbz|  PDB=3mbz  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mbz OCA], [https://pdbe.org/3mbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mbz RCSB], [https://www.ebi.ac.uk/pdbsum/3mbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mbz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RLA6_ACIBA Q8RLA6_ACIBA]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Class D beta-lactamases represent a growing and diverse class of penicillin-inactivating enzymes that are usually resistant to commercial beta-lactamase inhibitors. As many such enzymes are found in multi-drug resistant (MDR) Acinetobacter baumannii and Pseudomonas aeruginosa, novel beta-lactamase inhibitors are urgently needed. Five unique 6-alkylidene-2'-substituted penicillanic acid sulfones (1-5) were synthesized and tested against OXA-24, a clinically important beta-lactamase that inactivates carbapenems and is found in A. baumannii. Based upon the roles Tyr112 and Met223 play in the OXA-24 beta-lactamase, we also engineered two variants (Tyr112Ala and Tyr112Ala,Met223Ala) to test the hypothesis that the hydrophobic tunnel formed by these residues influences inhibitor recognition. IC(50) values against OXA-24 and two OXA-24 beta-lactamase variants ranged from 10 +/- 1 (4 vs WT) to 338 +/- 20 nM (5 vs Tyr112Ala, Met223Ala). Compound 4 possessed the lowest K(i) (500 +/- 80 nM vs WT), and 1 possessed the highest inactivation efficiency (k(inact)/K(i) = 0.21 +/- 0.02 muM(-1) s(-1)). Electrospray ionization mass spectrometry revealed a single covalent adduct, suggesting the formation of an acyl-enzyme intermediate. X-ray structures of OXA-24 complexed to four inhibitors (2.0-2.6 A) reveal the formation of stable bicyclic aromatic intermediates with their carbonyl oxygen in the oxyanion hole. These data provide the first structural evidence that 6-alkylidene-2'-substituted penicillin sulfones are effective mechanism-based inactivators of class D beta-lactamases. Their unique chemistry makes them developmental candidates. Mechanisms for class D hydrolysis and inhibition are discussed, and a pathway for the evolution of the BlaR1 sensor of Staphylococcus aureus to the class D beta-lactamases is proposed.


===OXA-24 beta-lactamase complex soaked with 10mM SA4-17 inhibitor for 15min===
Design, synthesis, and crystal structures of 6-alkylidene-2'-substituted penicillanic acid sulfones as potent inhibitors of Acinetobacter baumannii OXA-24 carbapenemase.,Bou G, Santillana E, Sheri A, Beceiro A, Sampson JM, Kalp M, Bethel CR, Distler AM, Drawz SM, Pagadala SR, van den Akker F, Bonomo RA, Romero A, Buynak JD J Am Chem Soc. 2010 Sep 29;132(38):13320-31. PMID:20822105<ref>PMID:20822105</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3mbz" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_20822105}}, adds the Publication Abstract to the page
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 20822105 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_20822105}}
__TOC__
 
</StructureSection>
==About this Structure==
[[3mbz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MBZ OCA].
 
==Reference==
<ref group="xtra">PMID:20822105</ref><references group="xtra"/>
[[Category: Acinetobacter baumannii]]
[[Category: Acinetobacter baumannii]]
[[Category: Beta-lactamase]]
[[Category: Large Structures]]
[[Category: Akker, F van den.]]
[[Category: Sampson J]]
[[Category: Sampson, J.]]
[[Category: Van den Akker F]]
Retrieved from "https://proteopedia.org/3mbz"