2p1l: Difference between revisions

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[[Image:2p1l.gif|left|200px]]<br /><applet load="2p1l" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2p1l, resolution 2.50&Aring;" />
'''Structure of the Bcl-XL:Beclin 1 complex'''<br />


==Overview==
==Structure of the Bcl-XL:Beclin 1 complex==
Bcl-2 family proteins are key regulators of apoptosis and have recently, been shown to modulate autophagy. The tumor suppressor Beclin 1 has been, proposed to coordinate both apoptosis and autophagy through direct, interaction with anti-apoptotic family members Bcl-2 and/or Bcl-X(L)., However, the molecular basis for this interaction remains enigmatic. Here, we report that Beclin 1 contains a conserved BH3 domain, which is both, necessary and sufficient for its interaction with Bcl-X(L). We also report, the crystal structure of a Beclin BH3 peptide in complex with Bcl-X(L) at, 2.5A resolution. Reminiscent of previously determined Bcl-X(L)-BH3, structures, the amphipathic BH3 helix of Beclin 1 bound to a conserved, hydrophobic groove of Bcl-X(L). These results define Beclin 1 as a novel, BH3-only protein, implying that Beclin 1 may have a direct role in, initiating apoptotic signaling. We propose that this putative apoptotic, function may be linked to the ability of Beclin 1 to suppress tumor, formation in mammals.
<StructureSection load='2p1l' size='340' side='right'caption='[[2p1l]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2p1l]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P1L FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p1l OCA], [https://pdbe.org/2p1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p1l RCSB], [https://www.ebi.ac.uk/pdbsum/2p1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p1l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref>  Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/2p1l_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p1l ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2P1L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P1L OCA].
*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of the Bcl-XL-Beclin 1 peptide complex: Beclin 1 is a novel BH3-only protein., Oberstein A, Jeffrey PD, Shi Y, J Biol Chem. 2007 Apr 27;282(17):13123-32. Epub 2007 Mar 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17337444 17337444]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Jeffrey, P.D.]]
[[Category: Jeffrey PD]]
[[Category: Oberstein, A.L.]]
[[Category: Oberstein AL]]
[[Category: Shi, Y.]]
[[Category: Shi Y]]
[[Category: apoptosis; autophagy; beclin; bh3 domain; bcl]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:59:19 2008''

Latest revision as of 09:07, 21 February 2024

Structure of the Bcl-XL:Beclin 1 complex

2p1l, resolution 2.50Å

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