2obu: Difference between revisions

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[[Image:2obu.gif|left|200px]]<br /><applet load="2obu" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2obu" />
'''Solution structure of GIP in TFE/water'''<br />


==Overview==
==Solution structure of GIP in TFE/water==
Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal, incretin hormone, which modulates physiological insulin secretion. Because, of its glucose-sensitive insulinotropic activity, there has been a, considerable interest in utilizing the hormone as a potential treatment, for type 2 diabetes. Structural parameters obtained from NMR spectroscopy, combined with molecular modeling techniques play a vital role in the, design of new therapeutic drugs. Therefore, to understand the structural, requirements for the biological activity of GIP, the solution structure of, GIP was investigated by circular dichroism (CD) followed by proton nuclear, magnetic resonance (NMR) spectroscopy. CD studies showed an increase in, the helical character of the peptide with increasing concentration of, trifluoroethanol (TFE) up to 50%. Therefore, the solution structure of GIP, in 50% TFE was determined. It was found that there was an alpha-helix, between residues 6 and 29, which tends to extend further up to residue 36., The implications of the C-terminal extended helical segment in the, inhibitory properties of GIP on gastric acid secretion are discussed. It, is shown that the adoption by GIP of an alpha-helical secondary structure, is a requirement for its biological activity. Knowledge of the solution, structure of GIP will help in the understanding of how the peptide, interacts with its receptor and aids in the design of new therapeutic, agents useful for the treatment of diabetes.
<StructureSection load='2obu' size='340' side='right'caption='[[2obu]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2obu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OBU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2obu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2obu OCA], [https://pdbe.org/2obu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2obu RCSB], [https://www.ebi.ac.uk/pdbsum/2obu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2obu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GIP_HUMAN GIP_HUMAN] Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal incretin hormone, which modulates physiological insulin secretion. Because of its glucose-sensitive insulinotropic activity, there has been a considerable interest in utilizing the hormone as a potential treatment for type 2 diabetes. Structural parameters obtained from NMR spectroscopy combined with molecular modeling techniques play a vital role in the design of new therapeutic drugs. Therefore, to understand the structural requirements for the biological activity of GIP, the solution structure of GIP was investigated by circular dichroism (CD) followed by proton nuclear magnetic resonance (NMR) spectroscopy. CD studies showed an increase in the helical character of the peptide with increasing concentration of trifluoroethanol (TFE) up to 50%. Therefore, the solution structure of GIP in 50% TFE was determined. It was found that there was an alpha-helix between residues 6 and 29, which tends to extend further up to residue 36. The implications of the C-terminal extended helical segment in the inhibitory properties of GIP on gastric acid secretion are discussed. It is shown that the adoption by GIP of an alpha-helical secondary structure is a requirement for its biological activity. Knowledge of the solution structure of GIP will help in the understanding of how the peptide interacts with its receptor and aids in the design of new therapeutic agents useful for the treatment of diabetes.


==About this Structure==
The bioactive conformation of glucose-dependent insulinotropic polypeptide by NMR and CD spectroscopy.,Alana I, Malthouse JP, O'Harte FP, Hewage CM Proteins. 2007 Jul 1;68(1):92-9. PMID:17393464<ref>PMID:17393464</ref>
2OBU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OBU OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The bioactive conformation of glucose-dependent insulinotropic polypeptide by NMR and CD spectroscopy., Alana I, Malthouse JP, O'Harte FP, Hewage CM, Proteins. 2007 Jul 1;68(1):92-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17393464 17393464]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 2obu" style="background-color:#fffaf0;"></div>
[[Category: Alana, I.]]
== References ==
[[Category: Harte, F.P.M.O.]]
<references/>
[[Category: Hewage, C.M.]]
__TOC__
[[Category: Malthouse, J.P.G.]]
</StructureSection>
[[Category: gip; nmr; molecular modelling; helix; diabetes; obesity]]
[[Category: Homo sapiens]]
 
[[Category: Large Structures]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:01:11 2008''
[[Category: Alana I]]
[[Category: Hewage CM]]
[[Category: Malthouse JPG]]
[[Category: O'Harte FPM]]
Retrieved from "https://proteopedia.org/2obu"