2b3b: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="2b3b" size="350" color="white" frame="true" align="right" spinBox="true" caption="2b3b, resolution 1.950Å" /> '''Thermus thermophilu...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2b3b.gif|left|200px]]<br /><applet load="2b3b" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2b3b, resolution 1.950&Aring;" />
'''Thermus thermophilus Glucose/Galactose Binding Protein With Bound Glucose'''<br />


==Overview==
==Thermus thermophilus Glucose/Galactose Binding Protein With Bound Glucose==
Periplasmic binding proteins (PBPs) comprise a protein superfamily that is, involved in prokaryotic solute transport and chemotaxis. These proteins, have been used to engineer reagentless biosensors to detect natural or, non-natural ligands. There is considerable interest in obtaining very, stable members of this superfamily from thermophilic bacteria to use as, robust engineerable parts in biosensor development. Analysis of the, recently determined genome sequence of Thermus thermophilus revealed the, presence of more than 30 putative PBPs in this thermophile. One of these, is annotated as a glucose binding protein (GBP) based on its genetic, linkage to genes that are homologous to an ATP-binding cassette glucose, transport system, although the PBP sequence is homologous to periplasmic, maltose binding proteins (MBPs). Here we present the cloning, over-expression, characterization of cognate ligands, and determination of, the X-ray crystal structure of this gene product. We find that it is a, very stable (apo-protein Tm value is 100(+/- 2) degrees C; complexes, 106(+/- 3) degrees C and 111(+/- 1) degrees C for glucose and galactose, respectively) glucose (Kd value is 0.08(+/- 0.03) microM) and galactose, (Kd value is 0.94(+/- 0.04) microM) binding protein. Determination of the, X-ray crystal structure revealed that this T. thermophilus glucose binding, protein (ttGBP) is structurally homologous to MBPs rather than other GBPs., The di or tri-saccharide ligands in MBPs are accommodated in long, relatively shallow grooves. In the ttGBP binding site, this groove is, partially filled by two loops and an alpha-helix, which create a buried, binding site that allows binding of only monosaccharides. Comparison of, ttGBP and MBP provides a clear example of structural adaptations by which, the size of ligand binding sites can be controlled in the PBP super, family.
<StructureSection load='2b3b' size='340' side='right'caption='[[2b3b]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2b3b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3B FirstGlance]. <br>
2B3B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=BGC:'>BGC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3B OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3b OCA], [https://pdbe.org/2b3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3b RCSB], [https://www.ebi.ac.uk/pdbsum/2b3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3b ProSAT]</span></td></tr>
The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites., Cuneo MJ, Changela A, Warren JJ, Beese LS, Hellinga HW, J Mol Biol. 2006 Sep 15;362(2):259-70. Epub 2006 Aug 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16904687 16904687]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Thermus thermophilus]]
[https://www.uniprot.org/uniprot/Q72KX2_THET2 Q72KX2_THET2]  
[[Category: Beese, L.S.]]
== Evolutionary Conservation ==
[[Category: Changela, A.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Cuneo, M.J.]]
Check<jmol>
[[Category: Hellinga, H.W.]]
  <jmolCheckbox>
[[Category: Warren, J.J.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3b_consurf.spt"</scriptWhenChecked>
[[Category: BGC]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: GLC]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: galactose]]
  </jmolCheckbox>
[[Category: glucose]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3b ConSurf].
[[Category: periplasmic binding protein]]
<div style="clear:both"></div>
[[Category: protein-carbohydrate complex]]
__TOC__
 
</StructureSection>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:02:45 2008''
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB27]]
[[Category: Beese LS]]
[[Category: Changela A]]
[[Category: Cuneo MJ]]
[[Category: Hellinga HW]]
[[Category: Warren JJ]]
Retrieved from "https://proteopedia.org/2b3b"