3rbt: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 3rbt is ON HOLD Authors: Bao-Yu Chen, Xiao-Xiao Ma, Xiang Tan, Jie-Pin Yang, Nan-Nan Zhang, Wei-Fang Li, Yuxing Chen, Qingyou Xia, Cong-Zhao Zhou D... |
No edit summary |
||
| (9 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal structure of glutathione S-transferase Omega 3 from the silkworm Bombyx mori== | |||
<StructureSection load='3rbt' size='340' side='right'caption='[[3rbt]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3rbt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RBT FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rbt OCA], [https://pdbe.org/3rbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rbt RCSB], [https://www.ebi.ac.uk/pdbsum/3rbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rbt ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q1HPV9_BOMMO Q1HPV9_BOMMO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Glutathione transferases (GSTs) are ubiquitous detoxification enzymes that conjugate hydrophobic xenobiotics with reduced glutathione. The silkworm Bombyx mori encodes four isoforms of GST Omega (GSTO), featured with a catalytic cysteine, except that bmGSTO3-3 has an asparagine substitution of this catalytic residue. Here, we determined the 2.20-A crystal structure of bmGSTO3-3, which shares a typical GST overall structure. However, the extended C-terminal segment that exists in all the four bmGSTOs occupies the G-site of bmGSTO3-3 and makes it unworkable, as shown by the activity assays. Upon mutation of Asn29 to Cys and truncation of the C-terminal segment, the in vitro GST activity of bmGSTO3-3 could be restored. These findings provided structural insights into the activity regulation of GSTOs. | |||
Structure-guided activity restoration of the silkworm glutathione transferase Omega GSTO3-3.,Chen BY, Ma XX, Guo PC, Tan X, Li WF, Yang JP, Zhang NN, Chen Y, Xia Q, Zhou CZ J Mol Biol. 2011 Sep 16;412(2):204-11. Epub 2011 Jul 22. PMID:21816159<ref>PMID:21816159</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3rbt" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bombyx mori]] | |||
[[Category: Large Structures]] | |||
[[Category: Chen B-Y]] | |||
[[Category: Chen Y]] | |||
[[Category: Li W-F]] | |||
[[Category: Ma X-X]] | |||
[[Category: Tan X]] | |||
[[Category: Xia Q]] | |||
[[Category: Yang J-P]] | |||
[[Category: Zhang N-N]] | |||
[[Category: Zhou C-Z]] | |||
