2p74: Difference between revisions

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New page: left|200px<br /><applet load="2p74" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p74, resolution 0.88Å" /> '''CTX-M-9 class A beta...
 
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[[Image:2p74.jpg|left|200px]]<br /><applet load="2p74" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2p74, resolution 0.88&Aring;" />
'''CTX-M-9 class A beta-lactamase apo crystal structure at 0.88 Angstrom resolution'''<br />


==Overview==
==CTX-M-9 class A beta-lactamase apo crystal structure at 0.88 Angstrom resolution==
The apo crystal structure of CTX-M-9 beta-lactamase has been determined to, 0.88 A at pH 8.8. This unusually clear picture of proton positions and, residue interactions supports the role of Glu166 as the general base for, the controversial acylation step of class A beta-lactamase catalysis. The, ability to distinguish low-energy conformations sampled by the enzyme, allows us to link the two conformations of Lys73 to different protonation, states of Glu166.
<StructureSection load='2p74' size='340' side='right'caption='[[2p74]], [[Resolution|resolution]] 0.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2p74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P74 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.88&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p74 OCA], [https://pdbe.org/2p74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p74 RCSB], [https://www.ebi.ac.uk/pdbsum/2p74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p74 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9L5C8_ECOLX Q9L5C8_ECOLX]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/2p74_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p74 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2P74 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P74 OCA].
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The Acylation Mechanism of CTX-M beta-Lactamase at 0.88 A Resolution., Chen Y, Bonnet R, Shoichet BK, J Am Chem Soc. 2007 Apr 5;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17408273 17408273]
[[Category: Beta-lactamase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bonnet, R.]]
[[Category: Bonnet R]]
[[Category: Chen, Y.]]
[[Category: Chen Y]]
[[Category: Shoichet, B.K.]]
[[Category: Shoichet BK]]
[[Category: K]]
[[Category: PO4]]
[[Category: acylation]]
[[Category: beta-lactamase]]
[[Category: ctx-m]]
[[Category: esbl]]
[[Category: ultra-high resolution]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:19:55 2008''

Latest revision as of 08:27, 30 October 2024

CTX-M-9 class A beta-lactamase apo crystal structure at 0.88 Angstrom resolution

2p74, resolution 0.88Å

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