2ong: Difference between revisions
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New page: left|200px<br /><applet load="2ong" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ong, resolution 2.7Å" /> '''Crystal Structure of ... |
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== | ==Crystal Structure of of limonene synthase with 2-fluorogeranyl diphosphate (FGPP).== | ||
The crystal structure of (4S)-limonene synthase from Mentha spic ata, a | <StructureSection load='2ong' size='340' side='right'caption='[[2ong]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2ong]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mentha_spicata Mentha spicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ONG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=FPG:(1S)-1-[(1R)-1-FLUOROETHYL]-1,5-DIMETHYLHEXYL+TRIHYDROGEN+DIPHOSPHATE'>FPG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ong FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ong OCA], [https://pdbe.org/2ong PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ong RCSB], [https://www.ebi.ac.uk/pdbsum/2ong PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ong ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q40322_MENSP Q40322_MENSP] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/2ong_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ong ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of (4S)-limonene synthase from Mentha spic ata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-A; resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction. | |||
Structure of limonene synthase, a simple model for terpenoid cyclase catalysis.,Hyatt DC, Youn B, Zhao Y, Santhamma B, Coates RM, Croteau RB, Kang C Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5360-5. Epub 2007 Mar 19. PMID:17372193<ref>PMID:17372193</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2ong" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mentha spicata]] | [[Category: Mentha spicata]] | ||
[[Category: Croteau R]] | |||
[[Category: Croteau | [[Category: Hyatt DC]] | ||
[[Category: Hyatt | [[Category: Kang C]] | ||
[[Category: Kang | [[Category: Youn B]] | ||
[[Category: Youn | |||
Latest revision as of 06:10, 1 October 2025
Crystal Structure of of limonene synthase with 2-fluorogeranyl diphosphate (FGPP).
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