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<StructureSection load='1oap' size='350' side='right' caption='E. coli pal protein periplasmic domain complex with sulfate (PDB entry [[1oap]])' scene=''> | |||
==Structure== | ==Structure== | ||
Pal is a lipoprotein which is anchored in the outer membrane with an approximate length of 173 amino acids in the precursor form. It contains a signal sequence including an LVAC motif, with the flexible, hydrophobic N-terminal tail binding to the inner leaflet of the outer membrane and anchoring it. During translocation, signal peptidase II cleaves the LVAC motif, and the localisation of Pal is dependent on the Lol protein system. Serine, the amino acid at #2 position in the mature protein is a localisation determinant for the position of Pal in the outer membrane<ref name='Godlewska'>PMID: 19519769</ref>. The periplasmic domain of Pal can be seen in the 3D structure 1OAP. | '''Pal (peptidoglycan-associated lipoprotein)''' is a lipoprotein which is anchored in the outer membrane with an approximate length of 173 amino acids in the precursor form. It contains a signal sequence including an LVAC motif, with the flexible, hydrophobic N-terminal tail binding to the inner leaflet of the outer membrane and anchoring it. During translocation, signal peptidase II cleaves the LVAC motif, and the localisation of Pal is dependent on the Lol protein system. Serine, the amino acid at #2 position in the mature protein is a localisation determinant for the position of Pal in the outer membrane<ref name='Godlewska'>PMID: 19519769</ref>. The periplasmic domain of Pal can be seen in the 3D structure 1OAP. | ||
==Function== | ==Function== | ||
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==Interactions of Pal== | ==Interactions of Pal== | ||
Directed deletion mutagenesis was carried out to investigate the interactions of Pal with different components of the Tol-Pal system<ref>PMID: 14731286</ref>: | Directed deletion mutagenesis was carried out to investigate the interactions of Pal with different components of the [[Tol]]-Pal system<ref>PMID: 14731286</ref>: | ||
#'''Pal and the peptidoglycan layer''' - specific residues in the C-terminal domain including S126, G128 and R146 are responsible for the modification of the electric charge, and may be responsible for affecting the peptidoglycan interaction. | #'''Pal and the peptidoglycan layer''' - specific residues in the C-terminal domain including S126, G128 and R146 are responsible for the modification of the electric charge, and may be responsible for affecting the peptidoglycan interaction. | ||
#'''Pal and TolB''' - ''see'' [[TolB]] | #'''Pal and TolB''' - ''see'' [[TolB]] | ||
#'''Pal and TolA''' - Pal may play a role in the stabilisation of [[TolA]], and that this interaction is essential if the cell integrity is to be maintained. | #'''Pal and TolA''' - Pal may play a role in the stabilisation of [[TolA]], and that this interaction is essential if the cell integrity is to be maintained. | ||
</StructureSection> | |||
==3D structures of Pal== | |||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | |||
[[1oap]] - EcPal periplasmic domain - ''Escherichia coli''<br /> | |||
[[9a2n]] – EcPal periplasmic domain - integrated<br /> | |||
[[2w8b]] – EcPal periplasmic domain + TolB <br /> | |||
[[9rle]], [[9rlf]], [[9rlk]], [[9rll]] – EcPal periplasmic domain + LOLC + LOLD + LOLE | |||
[[2hqs]] – EcPal + TolB<br /> | |||
[[2gnm]], [[2gnt]] - PaPal – ''Pterocarpus angolensis''<br /> | |||
[[1n3o]] – PaPal + methyl-glucose <br /> | |||
[[1n3p]] – PaPal + sucrose<br /> | |||
[[1n3q]] – PaPal + turanose<br /> | |||
[[1q8o]], [[1q8p]], [[1q8q]], [[1q8s]], [[1qmv]], [[1ukg]], [[2ar6]], [[2arb]], [[2are]], [[2arx]], [[2gn3]], [[2phr]], [[2phw]], [[2phx]] – PaPal + oligosaccharide<br /> | |||
[[4pwt]] – YpPal – ''Yersinia pestis''<br /> | |||
[[4r40]] – YpPal + TolB<br /> | |||
[[6bwl]] – Pal – ''Bacillus thuringiensis''<br /> | |||
[[5n2c]] – BcPal – ''Burkholderia cenocepcia''<br /> | |||
[[5lkw]] – BcPal + pimelic acid derivative<br /> | |||
[[4b5c]] – Pal – ''Burkholderia pseudomallei''<br /> | |||
[[8yzn]] – Pal – ''Pseudomonas alcaligenes''<br /> | |||
==References== | ==References== | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | |||
