Tol: Difference between revisions
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===Interaction of Tol with Colicins=== | ===Interaction of Tol with Colicins=== | ||
<structure load='2ivz' size='300' align='right' caption='Interaction of TolB and Colicin E9' (PDB entry [[2ivz]] | SCENE= Tol/Tolbcol/1 /> | <structure load='2ivz' size='300' align='right' caption='Interaction of TolB and Colicin E9 [[2ivz]]' (PDB entry [[2ivz]] | SCENE= Tol/Tolbcol/1 /> | ||
It is interesting to note that the name 'Tol' derives from experiments which show that the ''tol'' mutant is tolerant to colicin action<ref name='Davies'>PMID: 1095546</ref>. | |||
The Tol-Pal system is used by group A colicins in order to translocate across the outer membrane, targeting mainly the inner membrane component TolA as well as TolQ and TolR. The colicins set up a translocon, constituting of the outer membrane receptor, translocator proteins and one or more periplasmic translocator proteins. The colicins recruit the Tol proteins using a Tol binding antigen, or epitope, which is embedded in the IUTD (Intrinsically Unstructured [[Translocation domain]]) found on the N-terminal (T-) domain of the colicin<ref>PMID: 21060316</ref>. | The Tol-Pal system is used by group A colicins in order to translocate across the outer membrane, targeting mainly the inner membrane component TolA as well as TolQ and TolR. The colicins set up a translocon, constituting of the outer membrane receptor, translocator proteins and one or more periplasmic translocator proteins. The colicins recruit the Tol proteins using a Tol binding antigen, or epitope, which is embedded in the IUTD (Intrinsically Unstructured [[Translocation domain]]) found on the N-terminal (T-) domain of the colicin<ref>PMID: 21060316</ref>. | ||
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The structure shows the interaction between TolB and the <scene name='Tol/Tolbcolclose/1'>translocation domain</scene> of [[Colicin E9]]. | The structure shows the interaction between TolB and the <scene name='Tol/Tolbcolclose/1'>translocation domain</scene> of [[Colicin E9]]. | ||
The Tol system can be exploited by the following group A colicins<ref name="Cascales">PMID: 17347522</ref><ref>PMID: 1095546</ref>: | The Tol system can be exploited by the following group A colicins<ref name="Cascales">PMID: 17347522</ref><ref name='Davies'>PMID: 1095546</ref>: | ||
*[[Colicin A]] | *[[Colicin A]] | ||
*[[Colicin E1]] | *[[Colicin E1]] | ||
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==Regulation== | ==Regulation== | ||
It has been shown that the regulation of Tol genes is linked to the cell envelope stability<ref name="Cascales">PMID: 17347522</ref>. In studies with E. coli, the ''tol-pal'' genes have been induced by RcsC in response to cell envelope stress<ref>PMID: 8821933</ref>. RcsC is a transmembrane sensor kinase, and along with the cytoplasmic response regulator RcsB, makes up the RcsBC regulatory system<ref>PMID: 16166540</ref>. RcsBC is involved in the regulation of ''cps'' genes which code for the biosynthsis machinery of a major component for the capsula, cholanic acid. | It has been shown that the regulation of Tol genes is linked to the cell envelope stability<ref name="Cascales">PMID: 17347522</ref>. In studies with E. coli, the ''tol-pal'' genes have been induced by RcsC in response to cell envelope stress<ref>PMID: 8821933</ref>. RcsC is a transmembrane sensor kinase, and along with the cytoplasmic response regulator RcsB, makes up the RcsBC regulatory system<ref>PMID: 16166540</ref>. RcsBC is involved in the regulation of ''cps'' genes which code for the biosynthsis machinery of a major component for the capsula, cholanic acid. | ||
==3D structures of Tol proteins== | |||
[[TolA]], [[TolB]], [[TolR]] | |||
== References== | == References== | ||
<references/> | <references/> | ||
