3pe7: Difference between revisions

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[[Image:3pe7.png|left|200px]]


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==Oligogalacturonate lyase in complex with manganese==
The line below this paragraph, containing "STRUCTURE_3pe7", creates the "Structure Box" on the page.
<StructureSection load='3pe7' size='340' side='right'caption='[[3pe7]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3pe7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica_subsp._enterocolitica_8081 Yersinia enterocolitica subsp. enterocolitica 8081]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PE7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
{{STRUCTURE_3pe7|  PDB=3pe7  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pe7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pe7 OCA], [https://pdbe.org/3pe7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pe7 RCSB], [https://www.ebi.ac.uk/pdbsum/3pe7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pe7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A1JMA5_YERE8 A1JMA5_YERE8]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Oligogalactuonate lyases (OGLs, now also classified as pectate lyase family 22) are cytoplasmic enzymes found in pectinolytic members of Enterobacteriaceae, such as the enteropathogen Yersinia enterocolitica. OGLs utilize a beta-elimination mechanism to preferentially catalyze the conversion of saturated and unsaturated digalacturonate (GalA2) into monogalaturonate (GalA) and the 4,5-unsaturated GalA-like molecule, DKI. To provide mechanistic insights into the specificity of this enzyme activity we have characterized the OGL from Y. enterocolitica, YeOGL, on oligogalacturonides and determined its three-dimensional X-ray structure to 1.65 Angstroms. The model contains a Mn2+ atom in the active site, which is coordinated by three histidines, one glutamine, and an acetate ion. The acetate mimics the binding of the uronate group of galactourono-configured substrates. These findings, in combination with enzyme kinetics and metal supplementation assays, provide a framework for modeling the active site architecture of OGL. This enzyme appears to contain a histidine for the abstraction of the lower case Greek alpha-proton in the -1 subsite, a residue that is highly conserved throughout the OGL family and represents a unique catalytic base among pectic active lyases. In addition we present a hypothesis for an emerging relationship observed between the cellular distribution of pectate lyase folding and their distinct metal coordination chemistries.


===Oligogalacturonate lyase in complex with manganese===
THE ACTIVE SITE OF OGL PROVIDES UNIQUE INSIGHTS INTO CYTOPLASMIC OLIGOGALACTURONATE BETA-ELIMINATION.,Abbott DW, Gilbert HJ, Boraston AB J Biol Chem. 2010 Sep 17. PMID:20851883<ref>PMID:20851883</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3pe7" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 20851883 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_20851883}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3pe7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica_subsp._enterocolitica Yersinia enterocolitica subsp. enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PE7 OCA].
[[Category: Yersinia enterocolitica subsp. enterocolitica 8081]]
 
[[Category: Abbott DW]]
==Reference==
[[Category: Boraston AB]]
<ref group="xtra">PMID:20851883</ref><references group="xtra"/>
[[Category: Gilbert HJ]]
[[Category: Yersinia enterocolitica subsp. enterocolitica]]
[[Category: Abbott, D W.]]
[[Category: Boraston, A B.]]
[[Category: Gilbert, H J.]]
Retrieved from "https://proteopedia.org/3pe7"