3pkq: Difference between revisions

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[[Image:3pkq.png|left|200px]]


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==Q83D Variant of S. Enterica RmlA with dGTP==
The line below this paragraph, containing "STRUCTURE_3pkq", creates the "Structure Box" on the page.
<StructureSection load='3pkq' size='340' side='right'caption='[[3pkq]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3pkq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PKQ FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
{{STRUCTURE_3pkq|  PDB=3pkq  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pkq OCA], [https://pdbe.org/3pkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pkq RCSB], [https://www.ebi.ac.uk/pdbsum/3pkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pkq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RMLA_SALTY RMLA_SALTY] Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Is also able to convert non natural substrates such as a wide array of alpha-D-hexopyranosyl, deoxy-alpha-D-glucopyranosyl, aminodeoxy-alpha-D-hexopyranosyl and acetamidodeoxy-alpha-D-hexopyranosyl phosphates to their corresponding dTDP- and UDP-nucleotide sugars.<ref>PMID:8382158</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Directed evolution is a valuable technique to improve enzyme activity in the absence of a priori structural knowledge, which can be typically enhanced via structure-guided strategies. In this study, a combination of both whole-gene error-prone polymerase chain reaction and site-saturation mutagenesis enabled the rapid identification of mutations that improved RmlA activity toward non-native substrates. These mutations have been shown to improve activities over 10-fold for several targeted substrates, including non-native pyrimidine- and purine-based NTPs as well as non-native d- and l-sugars (both alpha- and beta-isomers). This study highlights the first broadly applicable high throughput sugar-1-phosphate nucleotidyltransferase screen and the first proof of concept for the directed evolution of this enzyme class toward the identification of uniquely permissive RmlA variants.


===Q83D Variant of S. Enterica RmlA with dGTP===
Expanding the Nucleotide and Sugar 1-Phosphate Promiscuity of Nucleotidyltransferase RmlA via Directed Evolution.,Moretti R, Chang A, Peltier-Pain P, Bingman CA, Phillips GN Jr, Thorson JS J Biol Chem. 2011 Apr 15;286(15):13235-43. Epub 2011 Feb 11. PMID:21317292<ref>PMID:21317292</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3pkq" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_21317292}}, adds the Publication Abstract to the page
*[[Glucose-1-phosphate thymidylyltransferase 3D structures|Glucose-1-phosphate thymidylyltransferase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 21317292 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21317292}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3pkq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKQ OCA].
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
 
[[Category: Bingman CA]]
==Reference==
[[Category: Chang A]]
<ref group="xtra">PMID:21317292</ref><references group="xtra"/>
[[Category: Moretti R]]
[[Category: Glucose-1-phosphate thymidylyltransferase]]
[[Category: Phillips Jr GN]]
[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
[[Category: Thorson JS]]
[[Category: Bingman, C A.]]
[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
[[Category: Chang, A.]]
[[Category: Moretti, R.]]
[[Category: Phillips, G N.]]
[[Category: Thorson, J S.]]
Retrieved from "https://proteopedia.org/3pkq"