3qns: Difference between revisions

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[[Image:3qns.jpg|left|200px]]


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==DyPB from Rhodococcus jostii RHA1, crystal form 2==
The line below this paragraph, containing "STRUCTURE_3qns", creates the "Structure Box" on the page.
<StructureSection load='3qns' size='340' side='right'caption='[[3qns]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3qns]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_jostii_RHA1 Rhodococcus jostii RHA1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QNS FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3qns|  PDB=3qns  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qns OCA], [https://pdbe.org/3qns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qns RCSB], [https://www.ebi.ac.uk/pdbsum/3qns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qns ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q0SE24_RHOJR Q0SE24_RHOJR]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The soil bacterium Rhodococcus jostii RHA1 contains two dye-decolorizing peroxidases (DyPs) named according to the subfamily they represent: DypA, predicted to be periplasmic, and DypB, implicated in lignin degradation. Steady-state kinetic studies of these enzymes revealed that they have much lower peroxidase activities than C- and D-type DyPs. Nevertheless, DypA showed 6-fold greater apparent specificity for the anthraquinone dye Reactive Blue 4 (k(cat)/K(m) = 12800 +/- 600 M(-1) s(-1)) than either ABTS or pyrogallol, consistent with previously characterized DyPs. By contrast, DypB showed the greatest apparent specificity for ABTS (k(cat)/K(m) = 2000 +/- 100 M(-1) s(-1)) and also oxidized Mn(II) (k(cat)/K(m) = 25.1 +/- 0.1 M(-1) s(-1)). Further differences were detected using electron paramagnetic resonance (EPR) spectroscopy: while both DyPs contained high-spin (S = (5)/(2)) Fe(III) in the resting state, DypA had a rhombic high-spin signal (g(y) = 6.32, g(x) = 5.45, and g(z) = 1.97) while DypB had a predominantly axial signal (g(y) = 6.09, g(x) = 5.45, and g(z) = 1.99). Moreover, DypA reacted with H(2)O(2) to generate an intermediate with features of compound II (Fe(IV) horizontal lineO). By contrast, DypB reacted with H(2)O(2) with a second-order rate constant of (1.79 +/- 0.06) x 10(5) M(-1) s(-1) to generate a relatively stable green-colored intermediate (t(1/2) approximately 9 min). While the electron absorption spectrum of this intermediate was similar to that of compound I of plant-type peroxidases, its EPR spectrum was more consistent with a poorly coupled protein-based radical than with an [Fe(IV) horizontal lineO Por(*)](+) species. The X-ray crystal structure of DypB, determined to 1.4 A resolution, revealed a hexacoordinated heme iron with histidine and a solvent species occupying axial positions. A solvent channel potentially provides access to the distal face of the heme for H(2)O(2). A shallow pocket exposes heme propionates to the solvent and contains a cluster of acidic residues that potentially bind Mn(II). Insight into the structure and function of DypB facilitates its engineering for the improved degradation of lignocellulose.


===DyPB from Rhodococcus jostii RHA1, crystal form 2===
Characterization of dye-decolorizing peroxidases from Rhodococcus jostii RHA1.,Roberts JN, Singh R, Grigg JC, Murphy ME, Bugg TD, Eltis LD Biochemistry. 2011 Jun 14;50(23):5108-19. Epub 2011 May 19. PMID:21534572<ref>PMID:21534572</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
[[3qns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_jostii_rha1 Rhodococcus jostii rha1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QNS OCA].
<div class="pdbe-citations 3qns" style="background-color:#fffaf0;"></div>
[[Category: Rhodococcus jostii rha1]]
== References ==
[[Category: Eltis, L D.]]
<references/>
[[Category: Grigg, J C.]]
__TOC__
[[Category: Murphy, M E.P.]]
</StructureSection>
[[Category: Roberts, J N.]]
[[Category: Large Structures]]
[[Category: Singh, R.]]
[[Category: Rhodococcus jostii RHA1]]
[[Category: Eltis LD]]
[[Category: Grigg JC]]
[[Category: Murphy MEP]]
[[Category: Roberts JN]]
[[Category: Singh R]]
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