1zxg: Difference between revisions
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New page: left|200px<br /><applet load="1zxg" size="350" color="white" frame="true" align="right" spinBox="true" caption="1zxg" /> '''Solution structure of A219'''<br /> ==Overv... |
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== | ==Solution structure of A219== | ||
We describe here the solution NMR structures of two IgG binding domains | <StructureSection load='1zxg' size='340' side='right'caption='[[1zxg]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1zxg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZXG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zxg OCA], [https://pdbe.org/1zxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zxg RCSB], [https://www.ebi.ac.uk/pdbsum/1zxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zxg ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q53759_STAAU Q53759_STAAU] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zx/1zxg_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zxg ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We describe here the solution NMR structures of two IgG binding domains with highly homologous sequences but different three-dimensional structures. The proteins, G311 and A219, are derived from the IgG binding domains of their wild-type counterparts, protein G and protein A, respectively. Through a series of site-directed mutations and phage display selections, the sequences of G311 and A219 were designed to converge to a point of high-level sequence identity while keeping their respective wild-type tertiary folds. Structures of both artificially evolved sequences were determined by NMR spectroscopy. The main chain fold of G311 can be superimposed on the wild-type alpha/beta protein G structure with a backbone rmsd of 1.4 A, and the A219 structure can be overlaid on the wild-type three-alpha-helix protein A fold also with a backbone rmsd of 1.4 A. The structure of G311, in particular, accommodates a large number of mutational changes without undergoing a change in the overall fold of the main chain. The structural differences are maintained despite a high level (59%) of sequence identity. These proteins serve as starting points for further experiments that will probe basic concepts of protein folding and conformational switching. | |||
Solution NMR structures of IgG binding domains with artificially evolved high levels of sequence identity but different folds.,He Y, Yeh DC, Alexander P, Bryan PN, Orban J Biochemistry. 2005 Nov 1;44(43):14055-61. PMID:16245921<ref>PMID:16245921</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1zxg" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
[[Category: Alexander | [[Category: Alexander P]] | ||
[[Category: Bryan | [[Category: Bryan PN]] | ||
[[Category: He | [[Category: He Y]] | ||
[[Category: Orban | [[Category: Orban J]] | ||
[[Category: Yeh | [[Category: Yeh DC]] | ||
Latest revision as of 08:13, 15 May 2024
Solution structure of A219
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