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New page: left|200px<br /><applet load="201d" size="350" color="white" frame="true" align="right" spinBox="true" caption="201d" /> '''SOLUTION STRUCTURE OF THE OXYTRICHA TELOMERI...
 
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[[Image:201d.jpg|left|200px]]<br /><applet load="201d" size="350" color="white" frame="true" align="right" spinBox="true"
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'''SOLUTION STRUCTURE OF THE OXYTRICHA TELOMERIC REPEAT D[G4(T4G4)3] G-TETRAPLEX'''<br />


==Overview==
==SOLUTION STRUCTURE OF THE OXYTRICHA TELOMERIC REPEAT D[G4(T4G4)3] G-TETRAPLEX==
The solution structure of Oxytricha telomere sequence d[G4(T4G4)3] in 0.1, M Na+ containing solution has been determined using a combined, NMR-molecular dynamics approach including relaxation matrix refinement., This four G4 repeat sequence folds intramolecularly into a right-handed, G-tetraplex containing four stacked G-tetrads which are connected by two, lateral T4 loops and a central diagonal T4 loop. The guanine glycosidic, bonds adopt a syn-anti alternation along the full length of the, d[G4(T4G4)3] sequence while the orientation around adjacent G-tetrads, switches between syn.syn.anti.anti and anti.anti.syn.syn alignments. Four, distinct grooves are formed by the parallel (two of medium width) and, anti-parallel (one wide and one narrow width) alignment of adjacent, G-G-G-G segments in the G-tetraplex. The T4 residues in the diagonal loop, are well-defined while the T4 residues in both lateral loops are, under-defined and sample multiple conformations. The solution structure of, the Na(+)-stabilized Oxytricha d[G4(T4G4)3] G-tetraplex and an earlier, solution structure reported from our laboratory on the Na(+)-stabilized, human d[AG3(T2AG3)3] G-tetraplex exhibit a common folding topology defined, by the same syn/anti distribution of guanine residues along individual, strands and around individual G-tetrads, as well as a common central, diagonal loop which defines the strand directionalities. The well-resolved, proton NMR spectra associated with the d[G4(T4G4)3] G-tetraplex opens the, opportunity for studies ranging from cation-dependent characterization of, G-tetraplex conformation and hydration to ligand and protein recognition, of the distinct grooves associated with this folding topology.
<StructureSection load='201d' size='340' side='right'caption='[[201d]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[201d]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=201D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=201D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=201d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=201d OCA], [https://pdbe.org/201d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=201d RCSB], [https://www.ebi.ac.uk/pdbsum/201d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=201d ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The solution structure of Oxytricha telomere sequence d[G4(T4G4)3] in 0.1 M Na+ containing solution has been determined using a combined NMR-molecular dynamics approach including relaxation matrix refinement. This four G4 repeat sequence folds intramolecularly into a right-handed G-tetraplex containing four stacked G-tetrads which are connected by two lateral T4 loops and a central diagonal T4 loop. The guanine glycosidic bonds adopt a syn-anti alternation along the full length of the d[G4(T4G4)3] sequence while the orientation around adjacent G-tetrads switches between syn.syn.anti.anti and anti.anti.syn.syn alignments. Four distinct grooves are formed by the parallel (two of medium width) and anti-parallel (one wide and one narrow width) alignment of adjacent G-G-G-G segments in the G-tetraplex. The T4 residues in the diagonal loop are well-defined while the T4 residues in both lateral loops are under-defined and sample multiple conformations. The solution structure of the Na(+)-stabilized Oxytricha d[G4(T4G4)3] G-tetraplex and an earlier solution structure reported from our laboratory on the Na(+)-stabilized human d[AG3(T2AG3)3] G-tetraplex exhibit a common folding topology defined by the same syn/anti distribution of guanine residues along individual strands and around individual G-tetrads, as well as a common central diagonal loop which defines the strand directionalities. The well-resolved proton NMR spectra associated with the d[G4(T4G4)3] G-tetraplex opens the opportunity for studies ranging from cation-dependent characterization of G-tetraplex conformation and hydration to ligand and protein recognition of the distinct grooves associated with this folding topology.


==About this Structure==
Solution structure of the Oxytricha telomeric repeat d[G4(T4G4)3] G-tetraplex.,Wang Y, Patel DJ J Mol Biol. 1995 Aug 4;251(1):76-94. PMID:7643391<ref>PMID:7643391</ref>
201D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=201D OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Solution structure of the Oxytricha telomeric repeat d[G4(T4G4)3] G-tetraplex., Wang Y, Patel DJ, J Mol Biol. 1995 Aug 4;251(1):76-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7643391 7643391]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 201d" style="background-color:#fffaf0;"></div>
[[Category: Patel, D.J.]]
== References ==
[[Category: Wang, Y.]]
<references/>
[[Category: dna]]
__TOC__
 
</StructureSection>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:46:45 2008''
[[Category: Large Structures]]
[[Category: Patel DJ]]
[[Category: Wang Y]]
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