296d: Difference between revisions

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New page: left|200px<br /><applet load="296d" size="350" color="white" frame="true" align="right" spinBox="true" caption="296d, resolution 2.250Å" /> '''SEQUENCE-DEPENDENT ...
 
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[[Image:296d.gif|left|200px]]<br /><applet load="296d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="296d, resolution 2.250&Aring;" />
'''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX'''<br />


==Overview==
==SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX==
The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the, dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved, by molecular replacement and refined to an R factor of 18.5% for 2125, reflections collected on a Xentronics area detector. The drug is bound in, the minor groove, at the five base-pair site 5'-ATTTG and is in a unique, orientation. This is displaced by one base pair in the 5' direction, compared to previously-determined structures of this drug with the, sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are, discussed in terms of several sequence-dependent structural features of, the DNA, with particular reference to differences in propeller twist and, minor-groove width.
<StructureSection load='296d' size='340' side='right'caption='[[296d]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[296d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=296D FirstGlance]. <br>
296D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=HT:'>HT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT:2-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>HT</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=296d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=296d OCA], [https://pdbe.org/296d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=296d RCSB], [https://www.ebi.ac.uk/pdbsum/296d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=296d ProSAT]</span></td></tr>
Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7515488 7515488]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Brown, D.G.]]
</StructureSection>
[[Category: Neidle, S.]]
[[Category: Large Structures]]
[[Category: Skelly, J.V.]]
[[Category: Brown DG]]
[[Category: Spink, N.]]
[[Category: Neidle S]]
[[Category: HT]]
[[Category: Skelly JV]]
[[Category: b-dna]]
[[Category: Spink N]]
[[Category: complexed with drug]]
[[Category: double helix]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:51:15 2008''

Latest revision as of 09:10, 14 February 2024

SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX

296d, resolution 2.25Å

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