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New page: left|200px<br /><applet load="2a6t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2a6t, resolution 2.50Å" /> '''Crystal structure of...
 
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[[Image:2a6t.gif|left|200px]]<br /><applet load="2a6t" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2a6t, resolution 2.50&Aring;" />
'''Crystal structure of S.pombe mRNA decapping enzyme Dcp2p'''<br />


==Overview==
==Crystal structure of S.pombe mRNA decapping enzyme Dcp2p==
Decapping is a key step in both general and nonsense-mediated 5' --&gt; 3', mRNA-decay pathways. Removal of the cap structure is catalyzed by the, Dcp1-Dcp2 complex. The crystal structure of a C-terminally truncated, Schizosaccharomyces pombe Dcp2p reveals two distinct domains: an, all-helical N-terminal domain and a C-terminal domain that is a classic, Nudix fold. The C-terminal domain of both Saccharomyces cerevisiae and S., pombe Dcp2p proteins is sufficient for decapping activity, although the, N-terminal domain can affect the efficiency of Dcp2p function. The binding, of Dcp2p to Dcp1p is mediated by a conserved surface on its N-terminal, domain, and the N-terminal domain is required for Dcp1p to stimulate Dcp2p, activity. The flexible nature of the N-terminal domain relative to the, C-terminal domain suggests that Dcp1p binding to Dcp2p may regulate Dcp2p, activity through conformational changes of the two domains.
<StructureSection load='2a6t' size='340' side='right'caption='[[2a6t]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2a6t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A6T FirstGlance]. <br>
2A6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6T OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6t OCA], [https://pdbe.org/2a6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a6t RCSB], [https://www.ebi.ac.uk/pdbsum/2a6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6t ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure and functional analysis of Dcp2p from Schizosaccharomyces pombe., She M, Decker CJ, Chen N, Tumati S, Parker R, Song H, Nat Struct Mol Biol. 2006 Jan;13(1):63-70. Epub 2005 Dec 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16341225 16341225]
== Function ==
[https://www.uniprot.org/uniprot/DCP2_SCHPO DCP2_SCHPO] Catalytic component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Decapping is the major pathway of mRNA degradation in yeast. It occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body.<ref>PMID:15671491</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/2a6t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a6t ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Single protein]]
[[Category: Chen N]]
[[Category: Chen, N.]]
[[Category: She M]]
[[Category: She, M.]]
[[Category: Song H]]
[[Category: Song, H.]]
[[Category: alpha/beta/alpha]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:55:51 2008''

Latest revision as of 13:40, 13 March 2024

Crystal structure of S.pombe mRNA decapping enzyme Dcp2p

2a6t, resolution 2.50Å

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