3rf9: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(6 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:3rf9.jpg|left|200px]]


<!--
==X-ray structure of RlmN from Escherichia coli==
The line below this paragraph, containing "STRUCTURE_3rf9", creates the "Structure Box" on the page.
<StructureSection load='3rf9' size='340' side='right'caption='[[3rf9]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3rf9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RF9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RF9 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
{{STRUCTURE_3rf9|  PDB=3rf9  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rf9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rf9 OCA], [https://pdbe.org/3rf9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rf9 RCSB], [https://www.ebi.ac.uk/pdbsum/3rf9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rf9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RLMN_ECOLI RLMN_ECOLI] Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity. Unmodified tRNA is not a suitable substrate for RlmN, which suggests that RlmN works in a late step during tRNA maturation.<ref>PMID:18025251</ref> <ref>PMID:21415317</ref> <ref>PMID:22891362</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The radical SAM (RS) enzymes RlmN and Cfr methylate 23S ribosomal RNA, modifying the C2 or C8 position of adenosine 2503. The methyl groups are installed by a two-step sequence involving initial methylation of a conserved Cys residue (RlmN Cys 355) by SAM. Methyl transfer to the substrate requires reductive cleavage of a second equivalent of SAM. Crystal structures of RlmN and RlmN with SAM show that a single molecule of SAM coordinates the [4Fe-4S] cluster. Residue Cys 355 is S-methylated and located proximal to the SAM methyl group, suggesting that SAM involved in the initial methyl transfer binds at the same site. Thus, RlmN accomplishes its complex reaction with structural economy, harnessing the two most important reactivities of SAM within a single site.


===X-ray structure of RlmN from Escherichia coli===
Structural Basis for Methyl Transfer by a Radical SAM Enzyme.,Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC Science. 2011 Apr 28. PMID:21527678<ref>PMID:21527678</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
[[3rf9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RF9 OCA].
<div class="pdbe-citations 3rf9" style="background-color:#fffaf0;"></div>
[[Category: Escherichia coli]]
== References ==
[[Category: Boal, A K.]]
<references/>
[[Category: Booker, S J.]]
__TOC__
[[Category: Grove, T L.]]
</StructureSection>
[[Category: McLaughlin, M I.]]
[[Category: Escherichia coli K-12]]
[[Category: Rosenzweig, A C.]]
[[Category: Large Structures]]
[[Category: Yennawar, N.]]
[[Category: Boal AK]]
[[Category: Booker SJ]]
[[Category: Grove TL]]
[[Category: McLaughlin MI]]
[[Category: Rosenzweig AC]]
[[Category: Yennawar N]]
Retrieved from "https://proteopedia.org/3rf9"