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| [[Image:2ana.jpg|left|200px]]<br /><applet load="2ana" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2ana, resolution 2.500Å" />
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| '''THE CRYSTAL STRUCTURE OF D(G-G-G-G-C-C-C-C). A MODEL FOR POLY(DG).POLY(DC)'''<br />
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| ==Overview== | | ==THE CRYSTAL STRUCTURE OF D(G-G-G-G-C-C-C-C). A MODEL FOR POLY(DG).POLY(DC)== |
| The structure of the DNA oligomer d(G-G-G-G-C-C-C-C) has been determined, at a resolution of 2.5 A by single-crystal X-ray methods. There are two, strands in the asymmetric unit, and these coil about each other to form a, right-handed double-helix of the A-type with Watson-Crick hydrogen bonds, between base-pairs. The helix has a shallow minor groove and a deep, water-filled major groove; almost all exposed functional groups on the DNA, are hydrated, and 106 ordered solvent molecules have been found. The two, d(G-G-G-G).d(C-C-C-C) segments in the octamer exhibit similar and uniform, structures, but there is a slight discontinuity at the GpC step between, them. A recurring feature of the structure is the overlap of adjacent, guanine bases in each GpG step, with the five-membered ring of one guanine, stacking on the six-membered ring of its neighbour. There is little or no, overlap between adjacent cytosine rings. Conformational parameters for, these GpG steps are compared with those from other single-crystal X-ray, analyses. In general, GpG steps exhibit high slide, low roll and variable, twist. Models for poly(dG).poly(dC) were generated by applying a simple, rotation and translation to each of the unmodified d(G-G-G-G).d(C-C-C-C), units. Detailed features of these models are shown to be compatible with, various assays of poly(dG).poly(dC) in solution, and are useful in, understanding the polymorphic behaviour of this sequence under a variety, of experimental conditions.
| | <StructureSection load='2ana' size='340' side='right'caption='[[2ana]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[2ana]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ANA FirstGlance]. <br> |
| 2ANA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANA OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ana FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ana OCA], [https://pdbe.org/2ana PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ana RCSB], [https://www.ebi.ac.uk/pdbsum/2ana PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ana ProSAT]</span></td></tr> |
| ==Reference==
| | </table> |
| The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC)., McCall M, Brown T, Kennard O, J Mol Biol. 1985 Jun 5;183(3):385-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=4020865 4020865]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Brown, T.]] | | [[Category: Large Structures]] |
| [[Category: Kennard, O.]] | | [[Category: Brown T]] |
| [[Category: McCall, M.]] | | [[Category: Kennard O]] |
| [[Category: a-dna]]
| | [[Category: McCall M]] |
| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:06:01 2008''
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