2b2c: Difference between revisions

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New page: left|200px<br /><applet load="2b2c" size="350" color="white" frame="true" align="right" spinBox="true" caption="2b2c, resolution 2.500Å" /> '''Cloning, expression...
 
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[[Image:2b2c.gif|left|200px]]<br /><applet load="2b2c" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2b2c, resolution 2.500&Aring;" />
'''Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase'''<br />


==Overview==
==Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase==
The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned, from the model nematode Caenorhabditis elegans. Biochemical, characterisation of the recombinantly expressed protein revealed a high, degree of similarity to other eukaryotic SPDS with the exception of a low, affinity towards the substrate decarboxylated S-adenosylmethionine (Km =, 110 microM) and a less pronounced feedback inhibition by the second, reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C., elegans protein that carries a nematode-specific insertion of 27 amino, acids close to its N-terminus was crystallized, leading to the first X-ray, structure of a dimeric eukaryotic SPDS.
<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [https://pdbe.org/2b2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [https://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G5EFG3_CAEEL G5EFG3_CAEEL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2c_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2c ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2B2C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Active as [http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA].
*[[Spermidine synthase 3D structures|Spermidine synthase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase., Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S, FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16226262 16226262]
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Spermidine synthase]]
[[Category: Al-Karadaghi S]]
[[Category: Al-Karadaghi, S.]]
[[Category: Dufe VT]]
[[Category: Dufe, V.T.]]
[[Category: Eschbach ML]]
[[Category: Eschbach, M.L.]]
[[Category: Haider N]]
[[Category: Haider, N.]]
[[Category: Karlberg T]]
[[Category: Karlberg, T.]]
[[Category: Luersen K]]
[[Category: Luersen, K.]]
[[Category: Walter RD]]
[[Category: Walter, R.D.]]
[[Category: beta-alpha]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:14:48 2008''

Latest revision as of 13:42, 13 March 2024

Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase

2b2c, resolution 2.50Å

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