3aix: Difference between revisions

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[[Image:3aix.jpg|left|200px]]


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==Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (I222)==
The line below this paragraph, containing "STRUCTURE_3aix", creates the "Structure Box" on the page.
<StructureSection load='3aix' size='340' side='right'caption='[[3aix]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3aix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3aix|  PDB=3aix  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aix OCA], [https://pdbe.org/3aix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aix RCSB], [https://www.ebi.ac.uk/pdbsum/3aix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aix ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PCNA3_SULTO PCNA3_SULTO] Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication (By similarity). Both trimeric complexes inhibit DNA ligase and both 3'-5' and 5'-3' activity of Hel308 (Hjm) helicase, but stimulate Hjc, the Holliday junction cleavage enzyme.[HAMAP-Rule:MF_00317]<ref>PMID:18782564</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Proliferating cell nuclear antigen (PCNA) is a key protein that orchestrates the arrangement of DNA-processing proteins on DNA during DNA metabolism. In crenarchaea, PCNA forms a heterotrimer (PCNA123) consisting of PCNA1, PCNA2, and PCNA3, while in most eukaryotes and many archaea PCNAs form a homotrimer. Interestingly, unique oligomeric PCNAs from Sulfolobus tokodaii were reported in which PCNA2 and PCNA3 form a heterotrimer without PCNA1. In this paper, we describe the crystal structure of the stoPCNA2-stoPCNA3 complex. While most DNA sliding clamps form ring-shaped structures, our crystal structure showed an elliptic ring-like heterotetrameric complex, differing from a previous reports. Furthermore, we investigated the composition and the dimension of the stoPCNA2-stoPCNA3 complex in the solution using gel-filtration column chromatography and small-angle X-ray scattering analyses, respectively. These results indicate that stoPCNA2 and stoPCNA3 form the heterotetramer in solution. Based on our heterotetrameric structure, we propose a possible biological role for the heterotetrameric complex as a Holliday junction clamp.


===Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (I222)===
A novel heterotetrameric structure of the crenarchaeal PCNA2-PCNA3 complex.,Kawai A, Hashimoto H, Higuchi S, Tsunoda M, Sato M, Nakamura KT, Miyamoto S J Struct Biol. 2011 Jun;174(3):443-50. Epub 2011 Feb 23. PMID:21352919<ref>PMID:21352919</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3aix" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_21352919}}, adds the Publication Abstract to the page
*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 21352919 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21352919}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3aix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIX OCA].
[[Category: Sulfurisphaera tokodaii str. 7]]
 
[[Category: Higuchi S]]
==Reference==
[[Category: Kawai A]]
<ref group="xtra">PMID:021352919</ref><references group="xtra"/>
[[Category: Miyamoto S]]
[[Category: Sulfolobus tokodaii]]
[[Category: Higuchi, S.]]
[[Category: Kawai, A.]]
[[Category: Miyamoto, S.]]

Latest revision as of 15:47, 4 October 2023

Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (I222)

3aix, resolution 2.90Å

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