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New page: left|200px<br /><applet load="2b5a" size="350" color="white" frame="true" align="right" spinBox="true" caption="2b5a, resolution 1.543Å" /> '''C.BclI, Control Ele...
 
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[[Image:2b5a.gif|left|200px]]<br /><applet load="2b5a" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2b5a, resolution 1.543&Aring;" />
'''C.BclI, Control Element of the BclI Restriction-Modification System'''<br />


==Overview==
==C.BclI, Control Element of the BclI Restriction-Modification System==
Protection from DNA invasion is afforded by restriction-modification, systems in many bacteria. The efficiency of protection depends crucially, on the relative expression levels of restriction versus methytransferase, genes. This regulation is provided by a controller protein, named C, protein. Studies of the Bcll system in E. coli suggest that C.Bcll, functions as a negative regulator for M.Bcll expression, implying that it, plays a role in defense against foreign DNA during virus infection. C.Bcll, binds (Kd = 14.3 nM) to a 2-fold symmetric C box DNA sequence that, overlaps with the putative -35 promoter region upstream of the bcllM and, bcllC genes. The C.Bcll fold comprises five alpha helices: two helices, form a helix-turn-helix motif, and the remaining three helices form the, extensive dimer interface. The C.Bcll-DNA model proposed suggests that DNA, bending might play an important role in gene regulation, and that Glu27, and Asp31 in C.Bcll might function critically in the regulation.
<StructureSection load='2b5a' size='340' side='right'caption='[[2b5a]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2b5a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_caldolyticus Bacillus caldolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5A FirstGlance]. <br>
2B5A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_caldolyticus Bacillus caldolyticus] with <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.543&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5a OCA], [https://pdbe.org/2b5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5a RCSB], [https://www.ebi.ac.uk/pdbsum/2b5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5a ProSAT]</span></td></tr>
Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site., Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK, Structure. 2005 Dec;13(12):1837-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16338412 16338412]
</table>
[[Category: Bacillus caldolyticus]]
== Evolutionary Conservation ==
[[Category: Protein complex]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Balendiran, G.K.]]
Check<jmol>
[[Category: Chan, S.H.]]
  <jmolCheckbox>
[[Category: Dabur, R.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b5a_consurf.spt"</scriptWhenChecked>
[[Category: Mersha, F.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Sawaya, M.R.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Xu, S.Y.]]
  </jmolCheckbox>
[[Category: Zhu, Z.]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b5a ConSurf].
[[Category: ACY]]
<div style="clear:both"></div>
[[Category: helix-turn-helix motif]]
__TOC__
 
</StructureSection>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:17:09 2008''
[[Category: Large Structures]]
[[Category: Balendiran GK]]
[[Category: Chan SH]]
[[Category: Dabur R]]
[[Category: Mersha F]]
[[Category: Sawaya MR]]
[[Category: Xu SY]]
[[Category: Zhu Z]]
Retrieved from "https://proteopedia.org/2b5a"