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New page: left|200px<br /><applet load="2bna" size="350" color="white" frame="true" align="right" spinBox="true" caption="2bna, resolution 2.700Å" /> '''STRUCTURE OF A B-DN...
 
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[[Image:2bna.jpg|left|200px]]<br /><applet load="2bna" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2bna, resolution 2.700&Aring;" />
'''STRUCTURE OF A B-DNA DODECAMER AT 16 KELVIN'''<br />


==Overview==
==STRUCTURE OF A B-DNA DODECAMER AT 16 KELVIN==
The crystal structure of the B-DNA dodecamer C-G-C-G-A-A-T-T-C-G-C-G, previously solved and refined at room temperature (290 K), has been, analyzed at 16 K (-257 degrees C). The end-to-end winding of the helix, does not vary with temperature but remains constant at 10.1 base pairs per, turn. Negatively charged phosphate groups throughout the structure do move, closer together on cooling, however, probably because of increase in the, dielectric constant of water as the temperature is lowered. This has the, two-fold effect of reducing the spacing between neighboring double helices, from 24.0 to 22.9 A and of narrowing the helix grooves within any isolated, molecule. Overall lattice displacements as deduced from crystallographic, temperature factors are very much decreased in the 16 K structure, yet, displacements at phosphates continue to exceed those of deoxyribose sugars, by B = 9 A2 and those of base pairs by B = 22 A2, even at this very low, temperature at which practically all thermal motion has been eliminated., These differences, formerly interpreted as evidence for thermal vibration, must now be attributed to static disorder.
<StructureSection load='2bna' size='340' side='right'caption='[[2bna]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2bna]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BNA FirstGlance]. <br>
2BNA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BNA OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bna OCA], [https://pdbe.org/2bna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bna RCSB], [https://www.ebi.ac.uk/pdbsum/2bna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bna ProSAT]</span></td></tr>
==Reference==
</table>
Structure of a B-DNA dodecamer at 16 K., Drew HR, Samson S, Dickerson RE, Proc Natl Acad Sci U S A. 1982 Jul;79(13):4040-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=6955789 6955789]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Dickerson, R.E.]]
[[Category: Large Structures]]
[[Category: Drew, H.R.]]
[[Category: Dickerson RE]]
[[Category: Samson, S.]]
[[Category: Drew HR]]
[[Category: b-dna]]
[[Category: Samson S]]
[[Category: double helix]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:27:35 2008''

Latest revision as of 09:17, 14 February 2024

STRUCTURE OF A B-DNA DODECAMER AT 16 KELVIN

2bna, resolution 2.70Å

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