2bv8: Difference between revisions
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New page: left|200px<br /><applet load="2bv8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2bv8, resolution 2.01Å" /> '''THE CRYSTAL STRUCTUR... |
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== | ==The crystal structure of Phycocyanin from Gracilaria chilensis.== | ||
Phycocyanin is a phycobiliprotein involved in light harvesting and | <StructureSection load='2bv8' size='340' side='right'caption='[[2bv8]], [[Resolution|resolution]] 2.01Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2bv8]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Agarophyton_chilense Agarophyton chilense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BV8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene>, <scene name='pdbligand=PEB:PHYCOERYTHROBILIN'>PEB</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bv8 OCA], [https://pdbe.org/2bv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bv8 RCSB], [https://www.ebi.ac.uk/pdbsum/2bv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bv8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6B8L6_GRATL Q6B8L6_GRATL] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bv/2bv8_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bv8 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23 degrees . The coordinates of a PC heterohexamer (alphabeta)(6) and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC ((I)beta(3)(82)-->(I)alpha(1)(84)-->(I)alpha(5)(84)-->(I)beta(6)(82) and (I)beta(3)(153)-->(I)beta(5)(153)) and two main inter PC ((I)beta(6)(82)-->(II)beta(3)(82) and (I)beta(5)(153)-->(II)beta(3)(153)) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex. | |||
The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC-PC complex.,Contreras-Martel C, Matamala A, Bruna C, Poo-Caamano G, Almonacid D, Figueroa M, Martinez-Oyanedel J, Bunster M Biophys Chem. 2007 Feb;125(2-3):388-96. Epub 2006 Nov 10. PMID:17118524<ref>PMID:17118524</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2bv8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Phycocyanin|Phycocyanin]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Agarophyton chilense]] | |||
[[Category: Large Structures]] | |||
[[Category: Bruna C]] | |||
[[Category: Bunster M]] | |||
[[Category: Contreras-Martel C]] | |||
[[Category: Martinez-Oyanedel J]] | |||
[[Category: Poo-Caama G]] | |||
Latest revision as of 13:56, 13 December 2023
The crystal structure of Phycocyanin from Gracilaria chilensis.
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