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[[Image:3riv.png|left|200px]]


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==The Crystal Structure of Leishmania major Peroxidase==
The line below this paragraph, containing "STRUCTURE_3riv", creates the "Structure Box" on the page.
<StructureSection load='3riv' size='340' side='right'caption='[[3riv]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3riv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major_strain_Friedlin Leishmania major strain Friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
{{STRUCTURE_3riv|  PDB=3riv  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3riv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riv OCA], [https://pdbe.org/3riv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3riv RCSB], [https://www.ebi.ac.uk/pdbsum/3riv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3riv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q4Q3K2_LEIMA Q4Q3K2_LEIMA]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The parasitic protozoa, Leishmania major, produces a peroxidase (abbreviated LmP) that exhibits activities characteristic of both yeast cytochrome c peroxidase (CCP) and plant cytosolic ascorbate peroxidase (APX). One common feature is a key Trp residue, Trp208 in LmP and Trp191 in CCP, that is situated adjacent to the proximal His heme ligand in CCP, APX and LmP. In CCP Trp191 forms a stable cationic radical after reaction with H2O2 to form Compound I; in APX the radical is located on the porphyrin ring. In order to clarify the role of Trp208 in LmP and to further probe peroxidase structure-function relationships we have determined the crystal structure of LmP and have studied the role of Trp208 using electron paramagnetic resonance spectroscopy (EPR), mutagenesis, and enzyme kinetics. Both CCP and LmP have an extended section of beta structure near Trp191 and 208, respectively, which is absent in APX. This region provides stability to the Trp191 radical in CCP. EPR of LmP Compound I exhibits an intense and stable signal similar to CCP Compound I. In the LmP Trp208Phe mutant this signal disappears indicating that Trp208 forms a stable cationic radical. In LmP conversion of the Cys197 to Thr significantly weakens the Compound I EPR signal and dramatically lowers enzyme activity. These results further support the view that modulation of the local electrostatic environment controls the stability of the Trp radical in peroxidases. Our results also suggest that the biological role of LmP is to function as a cytochrome c peroxdase.


===The Crystal Structure of Leishmania major Peroxidase===
The crystal structure of Leishmania major peroxidase and characterization of the compound I tryptophan radical.,Jasion VS, Polanco JA, Meharenna YT, Li H, Poulos TL J Biol Chem. 2011 May 13. PMID:21566139<ref>PMID:21566139</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3riv" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_21566139}}, adds the Publication Abstract to the page
*[[Ascorbate peroxidase 3D structures|Ascorbate peroxidase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 21566139 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_21566139}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3riv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIV OCA].
[[Category: Leishmania major strain Friedlin]]
 
[[Category: Jasion VS]]
==Reference==
[[Category: Li H]]
<ref group="xtra">PMID:021566139</ref><references group="xtra"/>
[[Category: Poulos TL]]
[[Category: L-ascorbate peroxidase]]
[[Category: Leishmania major]]
[[Category: Jasion, V S.]]
[[Category: Li, H.]]
[[Category: Poulos, T L.]]

Latest revision as of 10:11, 13 August 2026

The Crystal Structure of Leishmania major Peroxidase

3riv, resolution 1.76Å

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