G3p: Difference between revisions
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==Overview== | ==Overview== | ||
Gene 3 protein (g3p pr pIII) is a minor coat protein found on the surface of filamentous bacteriophage <ref name="lubkowski"> PMID:9461080 </ref>. The protein consists of 406 amino acids divided into three domains interspaced with glycine linkers <ref name="lubkowski"/> <ref name="cabilly"> PMID:10596371 </ref>. Peptides or proteins can be fused to g3p and evaluated for binding or other properties. | '''Gene 3 protein''' (g3p pr pIII) is a '''minor coat protein''' found on the surface of filamentous bacteriophage <ref name="lubkowski"> PMID:9461080 </ref>. The protein consists of 406 amino acids divided into three domains interspaced with glycine linkers <ref name="lubkowski"/> <ref name="cabilly"> PMID:10596371 </ref>. Peptides or proteins can be fused to g3p and evaluated for binding or other properties. | ||
==Structural Analysis== | ==Structural Analysis== | ||
<applet load='1g3p' size='350' frame='true' align='right' caption='D1 and D2 domains of g3p [[1g3p]]' /> | <applet load='1g3p' size='350' frame='true' align='right' caption='D1 and D2 domains of g3p complex with sulfate [[1g3p]]' /> | ||
Five major papers will be discussed outlining the evolution of structure analysis of g3p. | Five major papers will be discussed outlining the evolution of structure analysis of g3p. | ||
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The function of D3 was elicited last. D3 domain “anchors” to F pilus and is necessary for phage packaging<ref name="holliger 99"/>. | The function of D3 was elicited last. D3 domain “anchors” to F pilus and is necessary for phage packaging<ref name="holliger 99"/>. | ||
===Phage Display=== | ===Phage Display=== | ||
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==3D structures of G3p== | ==3D structures of G3p== | ||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | |||
[[2x9b]] – IF1G3p TolA-binding domain – Enterobacteria phage IF1<br /> | [[2x9b]] – IF1G3p TolA-binding domain – Enterobacteria phage IF1<br /> | ||
[[3knq]] - FDG3p | [[2x9a]] – IF1G3p TolA-binding domain + TolA C-terminal domain<br /> | ||
[[3dgs]] - FDG3p | [[3knq]], [[2g3p]] - FDG3p N-terminal – Enterobacteria phage FD<br /> | ||
[[ | [[3dgs]] - FDG3p N-terminal (mutant)<br /> | ||
[[ | [[1fgp]] – FDG3p membrane penetration domain 20-85 - NMR<br /> | ||
[[9g8e]] – FDG3p N2 domain 120-223 - NMR<br /> | |||
[[8b3o]] – F1G3p + G8p + G6p – Enterobacteria phage F1 – Cryo EM<br /> | |||
[[8ixk]], [[8jwx]] – M13G3p + G8p + G6p – Inovirus M13 – Cryo EM<br /> | |||
[[1tol]] – M13G3p N-terminal domain/ M13TolA C-terminal domain – Enterobacteria phage M13<br /> | [[1tol]] – M13G3p N-terminal domain/ M13TolA C-terminal domain – Enterobacteria phage M13<br /> | ||
[[1g3p]] - M13G3p N-terminal domain<br /> | [[1g3p]] - M13G3p N-terminal domain<br /> | ||
[[ | [[4eo0]], [[4eo1]] - IKEG3p pilus-binding domain – Enterobacteria phage IKE | ||
==References== | ==References== | ||
