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[[Image:2xns.jpg|left|200px]]


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==Crystal Structure Of Human G alpha i1 Bound To A Designed Helical Peptide Derived From The Goloco Motif Of RGS14==
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<StructureSection load='2xns' size='340' side='right'caption='[[2xns]], [[Resolution|resolution]] 3.41&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2xns]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XNS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.41&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
{{STRUCTURE_2xns|  PDB=2xns  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xns OCA], [https://pdbe.org/2xns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xns RCSB], [https://www.ebi.ac.uk/pdbsum/2xns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xns ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The de novo design of protein-binding peptides is challenging because it requires the identification of both a sequence and a backbone conformation favorable for binding. We used a computational strategy that iterates between structure and sequence optimization to redesign the C-terminal portion of the RGS14 GoLoco motif peptide so that it adopts a new conformation when bound to Galpha(i1). An X-ray crystal structure of the redesigned complex closely matches the computational model, with a backbone root-mean-square deviation of 1.1 A.


===CRYSTAL STRUCTURE OF HUMAN G ALPHA I1 BOUND TO A DESIGNED HELICAL PEPTIDE DERIVED FROM THE GOLOCO MOTIF OF RGS14===
Computational design of the sequence and structure of a protein-binding peptide.,Sammond DW, Bosch DE, Butterfoss GL, Purbeck C, Machius M, Siderovski DP, Kuhlman B J Am Chem Soc. 2011 Mar 30;133(12):4190-2. Epub 2011 Mar 9. PMID:21388199<ref>PMID:21388199</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2xns" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
{{ABSTRACT_PUBMED_21388199}}
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</StructureSection>
==About this Structure==
[[2xns]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XNS OCA].
 
==Reference==
<ref group="xtra">PMID:021388199</ref><ref group="xtra">PMID:017603074</ref><ref group="xtra">PMID:011976690</ref><references group="xtra"/>
[[Category: Heterotrimeric G-protein GTPase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Bosch, D.]]
[[Category: Large Structures]]
[[Category: Butterfoss, G L.]]
[[Category: Bosch D]]
[[Category: Kuhlman, B.]]
[[Category: Butterfoss GL]]
[[Category: Machius, M.]]
[[Category: Kuhlman B]]
[[Category: Sammond, D W.]]
[[Category: Machius M]]
[[Category: Siderovski, D P.]]
[[Category: Sammond DW]]
[[Category: Siderovski DP]]
Retrieved from "https://proteopedia.org/2xns"