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New page: left|200px<br /><applet load="2fs5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fs5, resolution 1.95Å" /> '''Crystal structure of...
 
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[[Image:2fs5.gif|left|200px]]<br /><applet load="2fs5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2fs5, resolution 1.95&Aring;" />
'''Crystal structure of TDP-fucosamine acetyltrasnferase (WecD)- apo form'''<br />


==Overview==
==Crystal structure of TDP-fucosamine acetyltransferase (WecD)- apo form==
Enterobacterial common antigen (ECA) is a polysaccharide found on the, outer membrane of virtually all gram-negative enteric bacteria and, consists of three sugars, N-acetyl-d-glucosamine, N-acetyl-d-mannosaminuronic acid, and 4-acetamido-4,6-dideoxy-d-galactose, organized into trisaccharide repeating units having the sequence, --&gt;3)-alpha-d-Fuc4NAc-(1--&gt;4)-beta-d-ManNAcA-(1--&gt;4)-alpha-d-GlcNAc-(1--&gt;., While the precise function of ECA is unknown, it has been linked to the, resistance of Shiga-toxin-producing Escherichia coli (STEC) O157:H7 to, organic acids and the resistance of Salmonella enterica to bile salts. The, final step in the synthesis of 4-acetamido-4,6-dideoxy-d-galactose, the, acetyl-coenzyme A (CoA)-dependent acetylation of the 4-amino group, is, carried out by TDP-fucosamine acetyltransferase (WecD). We have determined, the crystal structure of WecD in apo form at a 1.95-Angstrom resolution, and bound to acetyl-CoA at a 1.66-Angstrom resolution. WecD is a dimeric, enzyme, with each monomer adopting the GNAT N-acetyltransferase fold, common to a number of enzymes involved in acetylation of histones, aminoglycoside antibiotics, serotonin, and sugars. The crystal structure, of WecD, however, represents the first structure of a GNAT family member, that acts on nucleotide sugars. Based on this cocrystal structure, we have, used flexible docking to generate a WecD-bound model of the, acetyl-CoA-TDP-fucosamine tetrahedral intermediate, representing the, structure during acetyl transfer. Our structural data show that WecD does, not possess a residue that directly functions as a catalytic base, although Tyr208 is well positioned to function as a general acid by, protonating the thiolate anion of coenzyme A.
<StructureSection load='2fs5' size='340' side='right'caption='[[2fs5]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2fs5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_CFT073 Escherichia coli CFT073]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FS5 FirstGlance]. <br>
2FS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS5 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fs5 OCA], [https://pdbe.org/2fs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2fs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fs5 ProSAT]</span></td></tr>
Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis., Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A, J Bacteriol. 2006 Aug;188(15):5606-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16855251 16855251]
</table>
[[Category: Escherichia coli]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/WECD_ECOL6 WECD_ECOL6] Catalyzes the acetylation of dTDP-fucosamine (dTDP-4-amino-4,6-dideoxy-D-galactose) to dTDP-Fuc4NAc, which is utilized in the biosynthesis of the enterobacterial common antigen (ECA).[HAMAP-Rule:MF_02027]<ref>PMID:16855251</ref>
[[Category: BSGI, Montreal-Kingston.Bacterial.Structural.Genomics.Initiative.]]
== Evolutionary Conservation ==
[[Category: Cygler, M.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Hung, M.N.]]
Check<jmol>
[[Category: Matte, A.]]
  <jmolCheckbox>
[[Category: Munger, C.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/2fs5_consurf.spt"</scriptWhenChecked>
[[Category: Nadeau, G.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Rangarajan, E.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Sulea, T.]]
  </jmolCheckbox>
[[Category: ZN]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fs5 ConSurf].
[[Category: acetyltransferase]]
<div style="clear:both"></div>
[[Category: bsgi]]
== References ==
[[Category: gnat fold]]
<references/>
[[Category: montreal-kingston bacterial structural genomics initiative]]
__TOC__
[[Category: structural genomics]]
</StructureSection>
 
[[Category: Escherichia coli CFT073]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:43:36 2008''
[[Category: Large Structures]]
[[Category: Cygler M]]
[[Category: Hung MN]]
[[Category: Matte A]]
[[Category: Munger C]]
[[Category: Nadeau G]]
[[Category: Rangarajan E]]
[[Category: Sulea T]]

Latest revision as of 09:25, 14 February 2024

Crystal structure of TDP-fucosamine acetyltransferase (WecD)- apo form

2fs5, resolution 1.95Å

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