2g0b: Difference between revisions

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New page: left|200px<br /><applet load="2g0b" size="350" color="white" frame="true" align="right" spinBox="true" caption="2g0b, resolution 3.000Å" /> '''The structure of Fe...
 
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[[Image:2g0b.gif|left|200px]]<br /><applet load="2g0b" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2g0b, resolution 3.000&Aring;" />
'''The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes'''<br />


==Overview==
==The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes==
Attempts to access antibiotics by capturing biosynthetic genes and, pathways directly from environmental DNA, which is overwhelmingly derived, from uncultured bacteria, have revealed a large and previously unknown, family of N-acyl amino acid synthases (NASs). The structure of the NAS, FeeM reveals structural similarity to the GCN5-related N-acyl transferases, and acylhomoserine lactone synthases. The overall structure has a central, beta sheet with alpha helices on both sides. A bound product at a cleft in, the beta sheet identifies the active site and the structural basis for, catalysis, and sequence conservation in this region indicates a bias for, recognition over speed. FeeM interacts with an acyl carrier protein, (FeeL), and the structure, mutagenesis, and enzymatic measurements reveal, that a small hydrophobic pocket in alpha helix 5 dominates binding of FeeM, to FeeL. The structural and mechanistic analyses suggest that the products, of FeeM could be bacterial signaling agents.
<StructureSection load='2g0b' size='340' side='right'caption='[[2g0b]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2g0b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G0B FirstGlance]. <br>
2G0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium] with <scene name='pdbligand=NLT:'>NLT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0B OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NLT:N-DODECANOYL-L-TYROSINE'>NLT</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0b OCA], [https://pdbe.org/2g0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g0b RCSB], [https://www.ebi.ac.uk/pdbsum/2g0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g0b ProSAT]</span></td></tr>
FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding., Van Wagoner RM, Clardy J, Structure. 2006 Sep;14(9):1425-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16962973 16962973]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/Q8KNZ7_9BACT Q8KNZ7_9BACT]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g0/2g0b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g0b ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Uncultured bacterium]]
[[Category: Uncultured bacterium]]
[[Category: Clardy, J.]]
[[Category: Clardy J]]
[[Category: Wagoner, R.M.Van.]]
[[Category: Van Wagoner RM]]
[[Category: NLT]]
[[Category: antibiotic synthase]]
[[Category: environmental dna]]
[[Category: n-acyl transferase]]
[[Category: protein-product complex]]
 
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