2r8a: Difference between revisions

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[[Image:2r8a.png|left|200px]]


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==Crystal structure of the long-chain fatty acid transporter FadL mutant delta N8==
The line below this paragraph, containing "STRUCTURE_2r8a", creates the "Structure Box" on the page.
<StructureSection load='2r8a' size='340' side='right'caption='[[2r8a]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2r8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R8A FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr>
{{STRUCTURE_2r8a|  PDB=2r8a  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r8a OCA], [https://pdbe.org/2r8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r8a RCSB], [https://www.ebi.ac.uk/pdbsum/2r8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r8a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FADL_ECOLI FADL_ECOLI] Involved in translocation of long-chain fatty acids across the outer membrane. It is a receptor for the bacteriophage T2. FadL may form a specific channel.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r8/2r8a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r8a ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ligand-gated channels, in which a substrate transport pathway is formed as a result of the binding of a small-molecule chemical messenger, constitute a diverse class of membrane proteins with important functions in prokaryotic and eukaryotic organisms. Despite their widespread nature, no ligand-gated channels have yet been found within the outer membrane (OM) of Gram-negative bacteria. Here we show, using in vivo transport assays, intrinsic tryptophan fluorescence and X-ray crystallography, that high-affinity (submicromolar) substrate binding to the OM long-chain fatty acid transporter FadL from Escherichia coli causes conformational changes in the N terminus that open up a channel for substrate diffusion. The OM long-chain fatty acid transporter FadL from E. coli is a unique paradigm for OM diffusion-driven transport, in which ligand gating within a beta-barrel membrane protein is a prerequisite for channel formation.


===Crystal structure of the long-chain fatty acid transporter FadL mutant delta N8===
Ligand-gated diffusion across the bacterial outer membrane.,Lepore BW, Indic M, Pham H, Hearn EM, Patel DR, van den Berg B Proc Natl Acad Sci U S A. 2011 May 18. PMID:21593406<ref>PMID:21593406</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_21593406}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2r8a" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 21593406 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21593406}}
__TOC__
 
</StructureSection>
==About this Structure==
[[2r8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R8A OCA].
 
==Reference==
<ref group="xtra">PMID:021593406</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Berg, B van den.]]
[[Category: Large Structures]]
[[Category: Hearn, E M.]]
[[Category: Hearn EM]]
[[Category: Indic, M.]]
[[Category: Indic M]]
[[Category: Lepore, B W.]]
[[Category: Lepore BW]]
[[Category: Patel, D R.]]
[[Category: Patel DR]]
[[Category: Beta barrel]]
[[Category: Van den Berg B]]
[[Category: Lipid transport]]
[[Category: Outer membrane protein]]
[[Category: Phage recognition]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Transport protein]]

Latest revision as of 11:49, 30 August 2023

Crystal structure of the long-chain fatty acid transporter FadL mutant delta N8

2r8a, resolution 3.00Å

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