1vt5: Difference between revisions
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New page: '''Unreleased structure''' The entry 1vt5 is ON HOLD Authors: Haran, T.E., Shakked, Z., Wang, A.H.-J., Rich, A. Description: THE CRYSTAL STRUCTURE OF D(CCCCGGGG): A NEW A-FORM VARIANT ... |
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The | ==THE CRYSTAL STRUCTURE OF D(CCCCGGGG): A NEW A-FORM VARIANT WITH AN EXTENDED BACKBONE CONFORMATION== | ||
<StructureSection load='1vt5' size='340' side='right'caption='[[1vt5]], [[Resolution|resolution]] 2.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1vt5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VT5 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vt5 OCA], [https://pdbe.org/1vt5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vt5 RCSB], [https://www.ebi.ac.uk/pdbsum/1vt5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vt5 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of d(CCCCGGGG) has been determined at a resolution of 2.25 A. The oligomers crystallize as A-DNA duplexes occupying crystallographic two-fold axes. The backbone conformation is, in general, similar to that observed in previously reported crystal structures of A-DNA fragments, except for the central linkage, where it adopts an extended structure resulting from all trans conformation at the P-O5'-C5'-C4' bonds. This type of conformation facilitates interstrand stacking between the guanines at the C-G site. The local helix twist at this step is very small (25 degrees) compared to an overall average of 33.5 degrees. The unique structure of the C-G base-pair step, namely the extended backbone and the distinct stacking geometry, may be an important feature in the recognition mechanism between double-stranded DNA molecules and restriction endonucleases such as Msp I, which cuts the sequence CCGG very specifically with a rate unaffected by neighboring base pairs. | |||
The crystal structure of d(CCCCGGGG): a new A-form variant with an extended backbone conformation.,Haran TE, Shakked Z, Wang AH, Rich A J Biomol Struct Dyn. 1987 Oct;5(2):199-217. PMID:3271472<ref>PMID:3271472</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1vt5" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Haran TE]] | |||
[[Category: Rich A]] | |||
[[Category: Shakked Z]] | |||
[[Category: Wang AH-J]] | |||
Latest revision as of 06:31, 19 July 2023
THE CRYSTAL STRUCTURE OF D(CCCCGGGG): A NEW A-FORM VARIANT WITH AN EXTENDED BACKBONE CONFORMATION
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