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New page: left|200px<br /><applet load="2hem" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hem" /> '''NMR structure and Mg2+ binding of an RNA seg...
 
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[[Image:2hem.gif|left|200px]]<br /><applet load="2hem" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2hem" />
'''NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit.'''<br />


==Overview==
==NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit.==
Helix 42 of Domain II of Escherichia coli 23S ribosomal RNA underlies the, L7/L12 stalk in the ribosome and may be significant in positioning this, feature relative to the rest of the 50S ribosomal subunit. Unlike the, Haloarcula marismortui and Deinococcus radiodurans examples, the lower, portion of helix 42 in E.coli contains two consecutive G*A oppositions, with both adenines on the same side of the stem. Herein, the structure of, an analog of positions 1037-1043 and 1112-1118 in the helix 42 region is, reported. NMR spectra and structure calculations support a cis, Watson-Crick/Watson-Crick (cis W.C.) G*A conformation for the tandem, (G*A)2 in the analog and a minimally perturbed helical duplex stem. Mg2+, titration studies imply that the cis W.C. geometry of the tandem (G*A)2, probably allows O6 of G20 and N1 of A4 to coordinate with a Mg2+ ion as, indicated by the largest chemical shift changes associated with the imino, group of G20 and the H8 of G20 and A4. A cross-strand bridging Mg2+, coordination has also been found in a different sequence context in the, crystal structure of H.marismortui 23S rRNA, and therefore it may be a, rare but general motif in Mg2+ coordination.
<StructureSection load='2hem' size='340' side='right'caption='[[2hem]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2hem]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HEM FirstGlance]. <br>
2HEM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HEM OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hem OCA], [https://pdbe.org/2hem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hem RCSB], [https://www.ebi.ac.uk/pdbsum/2hem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hem ProSAT]</span></td></tr>
==Reference==
</table>
NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit., Zhao Q, Nagaswamy U, Lee H, Xia Y, Huang HC, Gao X, Fox GE, Nucleic Acids Res. 2005 Jun 6;33(10):3145-53. Print 2005. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15939932 15939932]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Fox, G.]]
[[Category: Large Structures]]
[[Category: Gao, X.]]
[[Category: Synthetic construct]]
[[Category: Lee, H.]]
[[Category: Fox G]]
[[Category: Nagaswamy, U.]]
[[Category: Gao X]]
[[Category: Xia, Y.]]
[[Category: Lee H]]
[[Category: Zhao, Q.]]
[[Category: Nagaswamy U]]
[[Category: 23s rrna]]
[[Category: Xia Y]]
[[Category: backbone dynamics]]
[[Category: Zhao Q]]
[[Category: cis watson-crick g a pair]]
[[Category: g a mismatch]]
[[Category: helix 42]]
[[Category: l7/l12 stalk]]
[[Category: mg2+ binding]]
[[Category: tandem (g a)2]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:19:28 2008''

Latest revision as of 06:40, 1 May 2024

NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit.

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