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New page: left|200px<br /><applet load="2ntz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ntz, resolution 3.35Å" /> '''Structure of a ParB-...
 
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[[Image:2ntz.jpg|left|200px]]<br /><applet load="2ntz" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ntz, resolution 3.35&Aring;" />
'''Structure of a ParB-DNA complex reveals a double B-box interaction'''<br />


==About this Structure==
==Structure of a ParB-DNA complex reveals a double B-box interaction==
2NTZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTZ OCA].  
<StructureSection load='2ntz' size='340' side='right'caption='[[2ntz]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
[[Category: Enterobacteria phage p21]]
== Structural highlights ==
[[Category: Single protein]]
<table><tr><td colspan='2'>[[2ntz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P1 Escherichia virus P1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTZ FirstGlance]. <br>
[[Category: Funnell, B.E.]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
[[Category: Mansoor, A.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
[[Category: Schumacher, M.A.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntz OCA], [https://pdbe.org/2ntz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntz RCSB], [https://www.ebi.ac.uk/pdbsum/2ntz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntz ProSAT]</span></td></tr>
[[Category: para]]
</table>
[[Category: parb]]
== Function ==
[[Category: partition]]
[https://www.uniprot.org/uniprot/Q38420_9CAUD Q38420_9CAUD]  
[[Category: segregation]]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ntz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The plasmid partition process is essential for plasmid propagation and is mediated by par systems, consisting of centromere-like sites and two proteins, ParA and ParB. In the first step of partition by the archetypical P1 system, ParB binds a complicated centromere-like site to form a large nucleoprotein segrosome. ParB is a dimeric DNA-binding protein that can bridge between both A-boxes and B-boxes located on the centromere. Its helix-turn-helix domains bind A-boxes and the dimer domain binds B-boxes. Binding of the first ParB dimer nucleates the remaining ParB molecules onto the centromere site, which somehow leads to the formation of a condensed segrosome superstructure. To further understand this unique DNA spreading capability of ParB, we crystallized and determined the structure of a 1:2 ParB-(142-333):A3-B2-box complex to 3.35A resolution. The structure reveals a remarkable four-way, protein-DNA bridged complex in which both ParB helix-turn-helix domains simultaneously bind adjacent A-boxes and the dimer domain bridges between two B-boxes. The multibridging capability and the novel dimer domain-B-box interaction, which juxtaposes the DNA sites close in space, suggests a mechanism for the formation of the wrapped solenoid-like segrosome superstructure. This multibridging capability of ParB is likely critical in its partition complex formation and pairing functions.


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:59:30 2008''
Structure of a four-way bridged ParB-DNA complex provides insight into P1 segrosome assembly.,Schumacher MA, Mansoor A, Funnell BE J Biol Chem. 2007 Apr 6;282(14):10456-64. Epub 2007 Feb 9. PMID:17293348<ref>PMID:17293348</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ntz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia virus P1]]
[[Category: Large Structures]]
[[Category: Funnell BE]]
[[Category: Mansoor A]]
[[Category: Schumacher MA]]
Retrieved from "https://proteopedia.org/2ntz"