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New page: left|200px<br /><applet load="2okh" size="350" color="white" frame="true" align="right" spinBox="true" caption="2okh, resolution 3.00Å" /> '''Crystal structure of...
 
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[[Image:2okh.gif|left|200px]]<br /><applet load="2okh" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2okh, resolution 3.00&Aring;" />
'''Crystal structure of dimeric form of PfFabZ in crystal form3'''<br />


==Overview==
==Crystal structure of dimeric form of PfFabZ in crystal form3==
The crystals obtained from various batches of crystallization trials of, FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of, the I222 cell showed a large variation of about 16 A, from c = 81 A to c =, 97 A. Complete data sets were collected for three crystal forms with, varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously., Here, the crystal structures of the other two crystal forms are reported, and a detailed structural comparison is made of the three crystal forms in, order to explore the possible reasons for the existence of, non-isomorphism. The conformations of three loops vary between the three, crystal forms. The disposition of the loops affects the crystal packing, and hence the unit-cell parameter. The crystallization condition and, crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that, pH-induced intrinsic conformational changes in the protein play a key role, in the observed differences.
<StructureSection load='2okh' size='340' side='right'caption='[[2okh]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2okh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OKH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2okh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2okh OCA], [https://pdbe.org/2okh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2okh RCSB], [https://www.ebi.ac.uk/pdbsum/2okh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2okh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q965D7_PLAFA Q965D7_PLAFA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/2okh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2okh ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.


==About this Structure==
Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum.,Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:17372349<ref>PMID:17372349</ref>
2OKH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OKH OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum., Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17372349 17372349]
</div>
<div class="pdbe-citations 2okh" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures|Beta-hydroxyacyl-acyl carrier protein dehydratase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
[[Category: Single protein]]
[[Category: Padala P]]
[[Category: Padala, P.]]
[[Category: Sharma SK]]
[[Category: Sharma, S.K.]]
[[Category: Suguna K]]
[[Category: Suguna, K.]]
[[Category: Surolia A]]
[[Category: Surolia, A.]]
[[Category: Surolia N]]
[[Category: Surolia, N.]]
[[Category: Swarnamukhi PL]]
[[Category: Swarnamukhi, P.L.]]
[[Category: fabz]]
[[Category: hotdog fold]]
[[Category: non-isomorphism]]
[[Category: plasmodium]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:12:29 2008''

Latest revision as of 08:57, 25 October 2023

Crystal structure of dimeric form of PfFabZ in crystal form3

2okh, resolution 3.00Å

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