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| [[Image:378d.gif|left|200px]]<br /><applet load="378d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="378d, resolution 2.400Å" />
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| '''STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA'''<br />
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| ==Overview== | | ==STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA== |
| The 2.40 A resolution crystal structure of a side-by-side binding of, distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended, alternating TA sequence has been determined. The unit-cell parameters are, a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous, side-by-side distamycin-DNA complexes which have only a single DNA strand, and one drug molecule in the asymmetric unit. The structure was solved by, the molecular-replacement method and refined to an R index of 21.0% using, 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules, bind two side-by-side antiparallel staggered distamycins spanning about, five base pairs and virtually covering the entire length of the DNA. The, octamer duplexes exhibit low-high alternations in the helical twist, sugar, puckering and the C-O3' and O3'-P torsion angles, similar to the earlier, side-by-side complexes containing inosine bases. The molecules are stacked, one over the other along the ac diagonal in an infinite pseudo-continuous, helical column with no lateral interactions.
| | <StructureSection load='378d' size='340' side='right'caption='[[378d]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[378d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=378D FirstGlance]. <br> |
| 378D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=DMY:'>DMY</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=378d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=378d OCA], [https://pdbe.org/378d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=378d RCSB], [https://www.ebi.ac.uk/pdbsum/378d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=378d ProSAT]</span></td></tr> |
| Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089456 10089456]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Mitra, S.N.]]
| | </StructureSection> |
| [[Category: Sundaralingam, M.]]
| | [[Category: Large Structures]] |
| [[Category: Wahl, M.C.]]
| | [[Category: Mitra SN]] |
| [[Category: DMY]]
| | [[Category: Sundaralingam M]] |
| [[Category: NA]] | | [[Category: Wahl MC]] |
| [[Category: double drug in minor groove]] | |
| [[Category: double helix]] | |
| [[Category: right handed dna]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:37:14 2008''
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