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New page: left|200px<br /><applet load="3bna" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bna, resolution 3.000Å" /> '''REVERSIBLE BENDING ...
 
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[[Image:3bna.gif|left|200px]]<br /><applet load="3bna" size="350" color="white" frame="true" align="right" spinBox="true"
caption="3bna, resolution 3.000&Aring;" />
'''REVERSIBLE BENDING AND HELIX GEOMETRY IN A B-DNA DODECAMER: CGCGAATTBRCGCG'''<br />


==Overview==
==REVERSIBLE BENDING AND HELIX GEOMETRY IN A B-DNA DODECAMER: CGCGAATTBRCGCG==
A double-helical B-DNA dodecamer has been analyzed by single crystal x-ray, diffraction methods and refined independently in four variants: sequence, CGCGAATTCGCG at 20 degrees C and at 16 K, and CGCGAATTBrCGCG in 60%, methylpentanediol at 20 and at 7 degrees C. The first three forms show a, 14-19 degrees bend in overall helix axis, but the fourth is straight and, unbent. Detailed comparisons of the various forms have led to a better, understanding of helix geometry and bending. Structural principles can be, understood best if organized under four headings: 1) intrinsic geometry of, the sugar rings, 2) stacking and relative motion of base pairs, 3), geometry of the connecting phosphate backbone, and 4) mechanics of bending, in B-DNA. The observed bending is neither completely localized nor smooth, and continuous, but an intermediate compromise that can be termed, "annealed kinking."
<StructureSection load='3bna' size='340' side='right'caption='[[3bna]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[3bna]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BNA FirstGlance]. <br>
3BNA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BNA OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBR:5-BROMO-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>CBR</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bna OCA], [https://pdbe.org/3bna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bna RCSB], [https://www.ebi.ac.uk/pdbsum/3bna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bna ProSAT]</span></td></tr>
Reversible bending and helix geometry in a B-DNA dodecamer: CGCGAATTBrCGCG., Fratini AV, Kopka ML, Drew HR, Dickerson RE, J Biol Chem. 1982 Dec 25;257(24):14686-707. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7174662 7174662]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Dickerson, R.E.]]
</StructureSection>
[[Category: Fratini, A.V.]]
[[Category: Large Structures]]
[[Category: Kopka, M.L.]]
[[Category: Dickerson RE]]
[[Category: b-dna]]
[[Category: Fratini AV]]
[[Category: double helix]]
[[Category: Kopka ML]]
[[Category: modified]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:39:14 2008''

Latest revision as of 09:58, 14 February 2024

REVERSIBLE BENDING AND HELIX GEOMETRY IN A B-DNA DODECAMER: CGCGAATTBRCGCG

3bna, resolution 3.00Å

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