3kvt: Difference between revisions

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New page: left|200px<br /><applet load="3kvt" size="350" color="white" frame="true" align="right" spinBox="true" caption="3kvt, resolution 2.00Å" /> '''TETRAMERIZATION DOMA...
 
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[[Image:3kvt.jpg|left|200px]]<br /><applet load="3kvt" size="350" color="white" frame="true" align="right" spinBox="true"
caption="3kvt, resolution 2.00&Aring;" />
'''TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL'''<br />


==Overview==
==TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL==
The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated, K+ channels encodes molecular determinants for subfamily-specific assembly, of alpha-subunits into functional tetrameric channels. Crystal structures, of T1 tetramers from Shaw and Shaker subfamilies reveal a common, four-layered scaffolding. Within layer 4, on the hypothetical, membrane-facing side of the tetramer, the Shaw T1 tetramer contains four, zinc ions; each is coordinated by a histidine and two cysteines from one, monomer and by one cysteine from an adjacent monomer. The amino acids, involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif, that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by, coimmunoprecipitation that a few characteristic residues in the subunit, interface are crucial for subfamily-specific tetramerization of the T1, domains.
<StructureSection load='3kvt' size='340' side='right'caption='[[3kvt]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3kvt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KVT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kvt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvt OCA], [https://pdbe.org/3kvt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kvt RCSB], [https://www.ebi.ac.uk/pdbsum/3kvt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kvt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O76457_APLCA O76457_APLCA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/3kvt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kvt ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
3KVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVT OCA].
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9886290 9886290]
[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bellamy, H.]]
[[Category: Bellamy H]]
[[Category: Bixby, K.A.]]
[[Category: Bixby KA]]
[[Category: Choe, S.]]
[[Category: Choe S]]
[[Category: Kreusch, A.]]
[[Category: Kreusch A]]
[[Category: Nanao, M.H.]]
[[Category: Nanao MH]]
[[Category: Pfaffinger, P.J.]]
[[Category: Pfaffinger PJ]]
[[Category: Shen, N.V.]]
[[Category: Shen NV]]
[[Category: ZN]]
[[Category: molecular recognition]]
[[Category: potassium channel]]
[[Category: tetramerization domain]]
[[Category: zinc-binding]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:40:44 2008''

Latest revision as of 10:18, 21 February 2024

TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL

3kvt, resolution 2.00Å

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