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New page: left|200px<br /><applet load="420d" size="350" color="white" frame="true" align="right" spinBox="true" caption="420d, resolution 1.900Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:420d.gif|left|200px]]<br /><applet load="420d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="420d, resolution 1.900&Aring;" />
'''CRYSTAL STRUCTURE OF A 16-MER RNA DUPLEX WITH NON-ADJACENT A(ANTI).G(SYN) MISMATCHES'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A 16-MER RNA DUPLEX WITH NON-ADJACENT A(ANTI).G(SYN) MISMATCHES==
G.A mispairs are one of the most common noncanonical structural motifs of, RNA. The 1.9 A resolution crystal structure of the RNA 16-mer, r(GCAGAGUUAAAUCUGC)2 has been determined with two isolated or nonadjacent, G.A mispairs. The molecule crystallizes with one duplex in the asymmetric, unit in space group R3 and unit cell dimensions a = b = c = 49.24 A and, alpha = beta = gamma = 51.2 degrees. It is the longest known, oligonucleotide duplex at this resolution and isomorphous to the 16-mer, duplex with the C.A+ mispairs [Pan, et al., (1998) J. Mol. Biol. 283, 977-984]. The C.A+ mispair behaves like a wobble pair while the G.A+ does, not. The G.A mispairs are protonated at N1 of the adenines as in the C.A+, mispairs, and two hydrogen bonds in the G(syn).A+(anti) conformation are, formed. The syn guanine is stabilized by an intranucleotide hydrogen bond, between the 2-amino and the 5'-phosphate groups. The G(syn).A+(anti), conformation can provide a different surface for recognition in the, grooves compared to other G.A hydrogen bonding schemes. The major groove, is widened between the two mispairs allowing access to ligands. One of the, 3-fold axes is occupied by a sodium ion and a water molecule, while a, second is occupied by another water molecule.
<StructureSection load='420d' size='340' side='right'caption='[[420d]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[420d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=420D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=420D FirstGlance]. <br>
420D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=420D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=420d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=420d OCA], [https://pdbe.org/420d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=420d RCSB], [https://www.ebi.ac.uk/pdbsum/420d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=420d ProSAT]</span></td></tr>
Crystal structure of an RNA 16-mer duplex R(GCAGAGUUAAAUCUGC)2 with nonadjacent G(syn).A+(anti) mispairs., Pan B, Mitra SN, Sundaralingam M, Biochemistry. 1999 Mar 2;38(9):2826-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10052954 10052954]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Mitra, S.N.]]
</StructureSection>
[[Category: Pan, B.]]
[[Category: Large Structures]]
[[Category: Sundaralingam, M.]]
[[Category: Mitra SN]]
[[Category: NA]]
[[Category: Pan B]]
[[Category: a-rna structure]]
[[Category: Sundaralingam M]]
[[Category: mismatch a rna]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:42:45 2008''

Latest revision as of 10:37, 1 March 2024

CRYSTAL STRUCTURE OF A 16-MER RNA DUPLEX WITH NON-ADJACENT A(ANTI).G(SYN) MISMATCHES

420d, resolution 1.90Å

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