2w7z: Difference between revisions

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[[Image:2w7z.png|left|200px]]


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==Structure of the pentapeptide repeat protein EfsQnr, a DNA gyrase inhibitor. Free amines modified by cyclic pentylation with glutaraldehyde.==
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<StructureSection load='2w7z' size='340' side='right'caption='[[2w7z]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2w7z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W7Z FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LP6:6-PIPERIDIN-1-YL-L-NORLEUCINE'>LP6</scene></td></tr>
{{STRUCTURE_2w7z|  PDB=2w7z  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w7z OCA], [https://pdbe.org/2w7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w7z RCSB], [https://www.ebi.ac.uk/pdbsum/2w7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w7z ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q837D8_ENTFA Q837D8_ENTFA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w7/2w7z_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w7z ConSurf].
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and the strong reducing agent borane-dimethylamine resulted in a derivatized protein which produced crystals that diffracted to 1.6 A resolution; their structure was subsequently determined by single-wavelength anomalous dispersion. Analysis of the derivatized protein using Fourier transform ion cyclotron resonance mass spectrometry indicated a mass increase of 68 Da per free amino group. Electron-density maps about a limited number of structurally ordered lysines indicated that the modification was a cyclic pentylation of free amines, producing piperidine groups.


===STRUCTURE OF THE PENTAPEPTIDE REPEAT PROTEIN EFSQNR, A DNA GYRASE INHIBITOR. FREE AMINES MODIFIED BY CYCLIC PENTYLATION WITH GLUTARALDEHYDE.===
Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.,Vetting MW, Hegde SS, Blanchard JS Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):462-9. Epub 2009, Apr 18. PMID:19390151<ref>PMID:19390151</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2w7z" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 19390151 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_19390151}}
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</StructureSection>
==About this Structure==
[[2w7z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA].
 
==Reference==
<ref group="xtra">PMID:019390151</ref><references group="xtra"/>
[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
[[Category: Blanchard, J S.]]
[[Category: Large Structures]]
[[Category: Hegde, S S.]]
[[Category: Blanchard JS]]
[[Category: Vetting, M W.]]
[[Category: Hegde SS]]
[[Category: Chemical modification]]
[[Category: Vetting MW]]
[[Category: Cyclic pentylation]]
[[Category: Glutaraldehyde]]
[[Category: Gyrase inhibitor]]
[[Category: Inhibitor]]
[[Category: Pentapeptide repeat protein]]
Retrieved from "https://proteopedia.org/2w7z"