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New page: left|200px<br /><applet load="446d" size="350" color="white" frame="true" align="right" spinBox="true" caption="446d, resolution 3.000Å" /> '''STRUCTURE OF THE OL...
 
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[[Image:446d.gif|left|200px]]<br /><applet load="446d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="446d, resolution 3.000&Aring;" />
'''STRUCTURE OF THE OLIGONUCLEOTIDE D(CGTATATACG) AS A SITE SPECIFIC COMPLEX WITH NICKEL IONS'''<br />


==Overview==
==STRUCTURE OF THE OLIGONUCLEOTIDE D(CGTATATACG) AS A SITE SPECIFIC COMPLEX WITH NICKEL IONS==
In this paper we explore the application of Ni2+to the crystallization of, oligonucleotides. We have determined in this way the structure of a fully, alternating (Y-R) decanucleotide d(CGTATATACG) by single crystal X-ray, diffraction. This is the first oligonucleotide crystal structure with an, alternating 5'-(TA)3-3' central part. Alternating oligonucleotides have a, particular interest since they often have a unique structure. In this case, the general conformation is B-like with an alternating twist and an, end-to-end interaction which involves terminal guanines. The crystal, belongs to space group P41212 with a = b = 52.46, c = 101.49 A. This, packing imposes a 90 degrees crossing of the symmetry related helices., This is a new way of packing for decamers.The oligonucleotide structure is, characterized by the specific association with seven nickel ions, involving the N7 atom of every guanine. One of the Ni2+ions is shared, between two guanines of symmetry related molecules. Until now no, oligonucleotide has been crystallized in the presence of this metal ion. A, novel C.A.T triplet structure has also been tentatively identified.
<StructureSection load='446d' size='340' side='right'caption='[[446d]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[446d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=446D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=446D FirstGlance]. <br>
446D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=446D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=446d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=446d OCA], [https://pdbe.org/446d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=446d RCSB], [https://www.ebi.ac.uk/pdbsum/446d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=446d ProSAT]</span></td></tr>
Structure of the oligonucleotide d(CGTATATACG) as a site-specific complex with nickel ions., Abrescia NG, Malinina L, Fernandez LG, Huynh-Dinh T, Neidle S, Subirana JA, Nucleic Acids Res. 1999 Apr 1;27(7):1593-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10075989 10075989]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Abrescia, N.G.A.]]
</StructureSection>
[[Category: Gonzaga, L.F.]]
[[Category: Large Structures]]
[[Category: Huynh-Dinh, T.]]
[[Category: Abrescia NGA]]
[[Category: Malinina, L.]]
[[Category: Gonzaga LF]]
[[Category: Neidle, S.]]
[[Category: Huynh-Dinh T]]
[[Category: Subirana, J.A.]]
[[Category: Malinina L]]
[[Category: NI]]
[[Category: Neidle S]]
[[Category: nickel binding]]
[[Category: Subirana JA]]
[[Category: triple helix]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:43:51 2008''

Latest revision as of 10:39, 1 March 2024

STRUCTURE OF THE OLIGONUCLEOTIDE D(CGTATATACG) AS A SITE SPECIFIC COMPLEX WITH NICKEL IONS

446d, resolution 3.00Å

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