2iz7: Difference between revisions

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[[Image:2iz7.png|left|200px]]


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==structure of Moco Carrier Protein from Chlamydomonas reinhardtii==
The line below this paragraph, containing "STRUCTURE_2iz7", creates the "Structure Box" on the page.
<StructureSection load='2iz7' size='340' side='right'caption='[[2iz7]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2iz7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IZ7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iz7 OCA], [https://pdbe.org/2iz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iz7 RCSB], [https://www.ebi.ac.uk/pdbsum/2iz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iz7 ProSAT]</span></td></tr>
{{STRUCTURE_2iz7|  PDB=2iz7  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RV61_CHLRE Q8RV61_CHLRE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iz/2iz7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iz7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The molybdenum cofactor (Moco) forms the catalytic site in all eukaryotic molybdenum enzymes and is synthesized by a multistep biosynthetic pathway. The mechanism of transfer, storage, and insertion of Moco into the appropriate apo-enzyme is poorly understood. In Chlamydomonas reinhardtii, a Moco carrier protein (MCP) has been identified and characterized recently. Here we show biochemical evidence that MCP binds Moco as well as the tungstate-substituted form of the cofactor (Wco) with high affinity, whereas molybdopterin, the ultimate cofactor precursor, is not bound. This binding selectivity points to a specific metal-mediated interaction with MCP, which protects Moco and Wco from oxidation with t((1/2)) of 24 and 96 h, respectively. UV-visible spectroscopy showed defined absorption bands at 393, 470, and 570 nm pointing to ene-diothiolate and protein side-chain charge transfer bonds with molybdenum. We have determined the crystal structure of MCP at 1.6 Angstrom resolution using seleno-methionated and native protein. The monomer constitutes a Rossmann fold with two homodimers forming a symmetrical tetramer in solution. Based on conserved surface residues, charge distribution, shape, in silico docking studies, structural comparisons, and identification of an anionbinding site, a prominent surface depression was proposed as a Moco-binding site, which was confirmed by structure-guided mutagenesis coupled to substrate binding studies.


===STRUCTURE OF MOCO CARRIER PROTEIN FROM CHLAMYDOMONAS REINHARDTII===
Function and structure of the molybdenum cofactor carrier protein from Chlamydomonas reinhardtii.,Fischer K, Llamas A, Tejada-Jimenez M, Schrader N, Kuper J, Ataya FS, Galvan A, Mendel RR, Fernandez E, Schwarz G J Biol Chem. 2006 Oct 6;281(40):30186-94. Epub 2006 Jul 27. PMID:16873364<ref>PMID:16873364</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_16873364}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2iz7" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 16873364 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_16873364}}
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</StructureSection>
==About this Structure==
[[2iz7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZ7 OCA].
 
==Reference==
<ref group="xtra">PMID:016873364</ref><references group="xtra"/>
[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Fernandez, E.]]
[[Category: Large Structures]]
[[Category: Fischer, K.]]
[[Category: Fernandez E]]
[[Category: Kuper, J.]]
[[Category: Fischer K]]
[[Category: Llamas, A.]]
[[Category: Kuper J]]
[[Category: Mendel, R R.]]
[[Category: Llamas A]]
[[Category: Schrader, N.]]
[[Category: Mendel RR]]
[[Category: Schwarz, G.]]
[[Category: Schrader N]]
[[Category: Tejada-Jimenez, M.]]
[[Category: Schwarz G]]
[[Category: Metal transport]]
[[Category: Tejada-Jimenez M]]
[[Category: Molybdenum cofactor]]
Retrieved from "https://proteopedia.org/2iz7"