3syn: Difference between revisions

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~~'''Unreleased structure'''~~

~~The entry~~ 3syn is ~~ON HOLD until Paper~~ Publication

==Crystal structure of FlhF in complex with its activator==

== Structural highlights ==

<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYN FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.063Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [https://pdbe.org/3syn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [https://www.ebi.ac.uk/pdbsum/3syn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.

== Publication Abstract from PubMed ==

Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete.

~~Authors:~~ Bange, G., ~~Kuemmerer~~, N., Wild, K., Sinning, I.

Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770<ref>PMID:22056770</ref>

~~Description~~: ~~Crystal structure of FlhF in complex with its activator~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

==See Also==

*[[Flagellar biosynthetic protein|Flagellar biosynthetic protein]]

*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]

*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]

== References ==

__TOC__

</StructureSection>

[[Category: Bacillus subtilis]]

[[Category: Large Structures]]

[[Category: Bange G]]

[[Category: Kuemmerer N]]

[[Category: Sinning I]]

[[Category: Wild K]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 3syn is ON HOLD  until Paper Publication
+==Crystal structure of FlhF in complex with its activator==
+<StructureSection load='3syn' size='340' side='right'caption='[[3syn]], [[Resolution|resolution]] 3.06&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYN FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.063&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [https://pdbe.org/3syn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [https://www.ebi.ac.uk/pdbsum/3syn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete.
-Authors: Bange, G., Kuemmerer, N., Wild, K., Sinning, I.
+Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770<ref>PMID:22056770</ref>
-Description: Crystal structure of FlhF in complex with its activator
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3syn" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Flagellar biosynthetic protein|Flagellar biosynthetic protein]]
+*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
+*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bacillus subtilis]]
+[[Category: Large Structures]]
+[[Category: Bange G]]
+[[Category: Kuemmerer N]]
+[[Category: Sinning I]]
+[[Category: Wild K]]