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New page: left|200px<br /><applet load="2qdq" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qdq, resolution 2.200Å" /> '''Crystal structure o...
 
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[[Image:2qdq.jpg|left|200px]]<br /><applet load="2qdq" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2qdq, resolution 2.200&Aring;" />
'''Crystal structure of the talin dimerisation domain'''<br />


==Overview==
==Crystal structure of the talin dimerisation domain==
Talin is a large dimeric protein that couples integrins to cytoskeletal, actin. Here, we report the structure of the C-terminal actin-binding, domain of talin, the core of which is a five-helix bundle linked to a, C-terminal helix responsible for dimerisation. The NMR structure of the, bundle reveals a conserved surface-exposed hydrophobic patch surrounded by, positively charged groups. We have mapped the actin-binding site to this, surface and shown that helix 1 on the opposite side of the bundle, negatively regulates actin binding. The crystal structure of the, dimerisation helix reveals an antiparallel coiled-coil with conserved, residues clustered on the solvent-exposed face. Mutagenesis shows that, dimerisation is essential for filamentous actin (F-actin) binding and, indicates that the dimerisation helix itself contributes to binding. We, have used these structures together with small angle X-ray scattering to, derive a model of the entire domain. Electron microscopy provides direct, evidence for binding of the dimer to F-actin and indicates that it binds, to three monomers along the long-pitch helix of the actin filament.
<StructureSection load='2qdq' size='340' side='right'caption='[[2qdq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2qdq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QDQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qdq OCA], [https://pdbe.org/2qdq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qdq RCSB], [https://www.ebi.ac.uk/pdbsum/2qdq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qdq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qd/2qdq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qdq ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2QDQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QDQ OCA].
*[[Talin 3D structures|Talin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The structure of the C-terminal actin-binding domain of talin., Gingras AR, Bate N, Goult BT, Hazelwood L, Canestrelli I, Grossmann JG, Liu H, Putz NS, Roberts GC, Volkmann N, Hanein D, Barsukov IL, Critchley DR, EMBO J. 2008 Jan 23;27(2):458-69. Epub 2007 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18157087 18157087]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Barsukov IL]]
[[Category: Barsukov, I.L.]]
[[Category: Bate N]]
[[Category: Bate, N.]]
[[Category: Critchley DRC]]
[[Category: Critchley, D.R.C.]]
[[Category: Gingras AR]]
[[Category: Gingras, A.R.]]
[[Category: Putz NSM]]
[[Category: Putz, N.S.M.]]
[[Category: abs3]]
[[Category: c-terminal actin binding site]]
[[Category: dimerisation domain]]
[[Category: latch domain]]
[[Category: structural protein]]
[[Category: talin]]
 
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