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[[Image:2qom.jpg|left|200px]]<br /><applet load="2qom" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2qom, resolution 2.66&Aring;" />
'''The crystal structure of the E.coli EspP autotransporter Beta-domain.'''<br />


==Overview==
==The crystal structure of the E.coli EspP autotransporter Beta-domain.==
Autotransporters are virulence factors produced by Gram-negative bacteria., They consist of two domains, an N-terminal 'passenger' domain and a, C-terminal beta-domain. beta-domains form beta-barrel structures in the, outer membrane while passenger domains are translocated into the, extracellular space. In some autotransporters, the two domains are, separated by proteolytic cleavage. Using X-ray crystallography, we solved, the 2.7-A structure of the post-cleavage state of the beta-domain of EspP, an autotransporter produced by Escherichia coli strain O157:H7. The, structure consists of a 12-stranded beta-barrel with the passenger, domain-beta-domain cleavage junction located inside the barrel pore, approximately midway between the extracellular and periplasmic surfaces of, the outer membrane. The structure reveals an unprecedented intra-barrel, cleavage mechanism and suggests that two conformational changes occur in, the beta-domain after cleavage, one conferring increased stability on the, beta-domain and another restricting access to the barrel pore.
<StructureSection load='2qom' size='340' side='right'caption='[[2qom]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2qom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QOM FirstGlance]. <br>
2QOM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOM OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qom OCA], [https://pdbe.org/2qom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qom RCSB], [https://www.ebi.ac.uk/pdbsum/2qom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qom ProSAT]</span></td></tr>
==Reference==
</table>
Autotransporter structure reveals intra-barrel cleavage followed by conformational changes., Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK, Nat Struct Mol Biol. 2007 Dec;14(12):1214-20. Epub 2007 Nov 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17994105 17994105]
== Function ==
[[Category: Escherichia coli]]
[https://www.uniprot.org/uniprot/ESPP_ECO57 ESPP_ECO57] Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.<ref>PMID:9194704</ref> <ref>PMID:15615856</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Barnard, T.J.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Bernstein, H.D.]]
Check<jmol>
[[Category: Buchanan, S.K.]]
  <jmolCheckbox>
[[Category: Dautin, N.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/2qom_consurf.spt"</scriptWhenChecked>
[[Category: Lukacik, P.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: autotransporter]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: beta-barrel]]
  </jmolCheckbox>
[[Category: beta-domain]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qom ConSurf].
[[Category: hydrolase]]
<div style="clear:both"></div>
[[Category: outer membrane protein]]
== References ==
[[Category: plasmid]]
<references/>
[[Category: protease]]
__TOC__
[[Category: secreted]]
</StructureSection>
[[Category: serine protease]]
[[Category: Escherichia coli O157:H7]]
[[Category: transmembrane]]
[[Category: Large Structures]]
[[Category: virulence]]
[[Category: Barnard TJ]]
[[Category: zymogen]]
[[Category: Bernstein HD]]
 
[[Category: Buchanan SK]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 10:59:13 2008''
[[Category: Dautin N]]
[[Category: Lukacik P]]

Latest revision as of 09:17, 21 February 2024

The crystal structure of the E.coli EspP autotransporter Beta-domain.

2qom, resolution 2.66Å

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