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[[Image:1ojl.gif|left|200px]]<br />
<applet load="1ojl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ojl, resolution 3.0&Aring;" />
'''CRYSTAL STRUCTURE OF A SIGMA54-ACTIVATOR SUGGESTS THE MECHANISM FOR THE CONFORMATIONAL SWITCH NECESSARY FOR SIGMA54 BINDING'''<br />


==Overview==
==Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding==
The sigma(54)-dependent transcription in bacteria is associated with, various stress and growth conditions. Activators of the sigma(54) protein, contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both, prokaryotic and eukaryotic cells. We describe the X-ray structure of an, N-terminal domain deletion of the ZraR protein from Salmonella, typhimurium, which is a homologue of the general nitrogen regulatory, protein NtrC, at 3A resolution. The structure reveals a hexameric ring, that is typical for AAA+ containing proteins but which differs from the, heptameric ring found in the crystal structure of the AAA+ domain of NtrC1, from Aquifex aeolicus. The dimerisation interface between DNA-binding, domains observed in ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16005641 (full description)]]
<StructureSection load='1ojl' size='340' side='right'caption='[[1ojl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ojl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OJL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ojl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ojl OCA], [https://pdbe.org/1ojl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ojl RCSB], [https://www.ebi.ac.uk/pdbsum/1ojl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ojl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ZRAR_SALTY ZRAR_SALTY] Member of the two-component regulatory system ZraS/ZraR. When activated by ZraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oj/1ojl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ojl ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The sigma(54)-dependent transcription in bacteria is associated with various stress and growth conditions. Activators of the sigma(54) protein contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both prokaryotic and eukaryotic cells. We describe the X-ray structure of an N-terminal domain deletion of the ZraR protein from Salmonella typhimurium, which is a homologue of the general nitrogen regulatory protein NtrC, at 3A resolution. The structure reveals a hexameric ring that is typical for AAA+ containing proteins but which differs from the heptameric ring found in the crystal structure of the AAA+ domain of NtrC1 from Aquifex aeolicus. The dimerisation interface between DNA-binding domains observed in the crystal structure suggests that dodecamers, rather than hexamers, might be the functionally important oligomer.


==About this Structure==
Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR.,Sallai L, Tucker PA J Struct Biol. 2005 Aug;151(2):160-70. PMID:16005641<ref>PMID:16005641</ref>
1OJL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with PO4 and ATP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OJL OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR., Sallai L, Tucker PA, J Struct Biol. 2005 Aug;151(2):160-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16005641 16005641]
</div>
[[Category: Salmonella typhimurium]]
<div class="pdbe-citations 1ojl" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Sallai, L.]]
<references/>
[[Category: Tucker, P.A.]]
__TOC__
[[Category: ATP]]
</StructureSection>
[[Category: PO4]]
[[Category: Large Structures]]
[[Category: aaa domain]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: dna-binding]]
[[Category: Sallai L]]
[[Category: ntrc family]]
[[Category: Tucker PA]]
[[Category: response regulator]]
[[Category: transcription regulation]]
[[Category: two component system]]
 
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Retrieved from "https://proteopedia.org/1ojl"