3zyj: Difference between revisions

Latest revision as of 11:16, 20 December 2023

NetrinG1 in complex with NGL1

Structural highlights

3zyj is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.25Å
Ligands:	BMA, CA, MAN, NAG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

NTNG1_HUMAN Atypical Rett syndrome.

Function

NTNG1_HUMAN Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.^[1]

Publication Abstract from PubMed

Brain wiring depends on cells making highly localized and selective connections through surface protein-protein interactions, including those between NetrinGs and NetrinG ligands (NGLs). The NetrinGs are members of the structurally uncharacterized netrin family. We present a comprehensive crystallographic analysis comprising NetrinG1-NGL1 and NetrinG2-NGL2 complexes, unliganded NetrinG2 and NGL3. Cognate NetrinG-NGL interactions depend on three specificity-conferring NetrinG loops, clasped tightly by matching NGL surfaces. We engineered these NGL surfaces to implant custom-made affinities for NetrinG1 and NetrinG2. In a cellular patterning assay, we demonstrate that NetrinG-binding selectivity can direct the sorting of a mixed population of NGLs into discrete cell surface subdomains. These results provide a molecular model for selectivity-based patterning in a neuronal recognition system, dysregulation of which is associated with severe neuropsychological disorders.

Structural basis for cell surface patterning through NetrinG-NGL interactions.,Seiradake E, Coles CH, Perestenko PV, Harlos K, McIlhinney RA, Aricescu AR, Jones EY EMBO J. 2011 Sep 23. doi: 10.1038/emboj.2011.346. PMID:21946559^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Seiradake E, Coles CH, Perestenko PV, Harlos K, McIlhinney RA, Aricescu AR, Jones EY. Structural basis for cell surface patterning through NetrinG-NGL interactions. EMBO J. 2011 Sep 23. doi: 10.1038/emboj.2011.346. PMID:21946559 doi:10.1038/emboj.2011.346
↑ Seiradake E, Coles CH, Perestenko PV, Harlos K, McIlhinney RA, Aricescu AR, Jones EY. Structural basis for cell surface patterning through NetrinG-NGL interactions. EMBO J. 2011 Sep 23. doi: 10.1038/emboj.2011.346. PMID:21946559 doi:10.1038/emboj.2011.346

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Seiradake E, Coles CH, Perestenko PV, Harlos K, McIlhinney RA, Aricescu AR, Jones EY. Structural basis for cell surface patterning through NetrinG-NGL interactions. EMBO J. 2011 Sep 23. doi: 10.1038/emboj.2011.346. PMID:21946559 doi:10.1038/emboj.2011.346

[2] Seiradake E, Coles CH, Perestenko PV, Harlos K, McIlhinney RA, Aricescu AR, Jones EY. Structural basis for cell surface patterning through NetrinG-NGL interactions. EMBO J. 2011 Sep 23. doi: 10.1038/emboj.2011.346. PMID:21946559 doi:10.1038/emboj.2011.346

[1]

[2]

@@ Line 1: / Line 1: @@
-[[Image:3zyj.jpg|left|200px]]
-<!--
+==NetrinG1 in complex with NGL1==
-The line below this paragraph, containing "STRUCTURE_3zyj", creates the "Structure Box" on the page.
+<StructureSection load='3zyj' size='340' side='right'caption='[[3zyj]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3zyj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZYJ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-{{STRUCTURE_3zyj|  PDB=3zyj  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyj OCA], [https://pdbe.org/3zyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zyj RCSB], [https://www.ebi.ac.uk/pdbsum/3zyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zyj ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/NTNG1_HUMAN NTNG1_HUMAN] Atypical Rett syndrome.
+== Function ==
+[https://www.uniprot.org/uniprot/NTNG1_HUMAN NTNG1_HUMAN] Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.<ref>PMID:21946559</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Brain wiring depends on cells making highly localized and selective connections through surface protein-protein interactions, including those between NetrinGs and NetrinG ligands (NGLs). The NetrinGs are members of the structurally uncharacterized netrin family. We present a comprehensive crystallographic analysis comprising NetrinG1-NGL1 and NetrinG2-NGL2 complexes, unliganded NetrinG2 and NGL3. Cognate NetrinG-NGL interactions depend on three specificity-conferring NetrinG loops, clasped tightly by matching NGL surfaces. We engineered these NGL surfaces to implant custom-made affinities for NetrinG1 and NetrinG2. In a cellular patterning assay, we demonstrate that NetrinG-binding selectivity can direct the sorting of a mixed population of NGLs into discrete cell surface subdomains. These results provide a molecular model for selectivity-based patterning in a neuronal recognition system, dysregulation of which is associated with severe neuropsychological disorders.
-===NETRING1 IN COMPLEX WITH NGL1===
+Structural basis for cell surface patterning through NetrinG-NGL interactions.,Seiradake E, Coles CH, Perestenko PV, Harlos K, McIlhinney RA, Aricescu AR, Jones EY EMBO J. 2011 Sep 23. doi: 10.1038/emboj.2011.346. PMID:21946559<ref>PMID:21946559</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3zyj" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[3zyj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYJ OCA].
+*[[Netrin|Netrin]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Aricescu, A R.]]
+[[Category: Large Structures]]
-[[Category: Coles, C H.]]
+[[Category: Aricescu AR]]
-[[Category: Harlos, K.]]
+[[Category: Coles CH]]
-[[Category: Jones, E Y.]]
+[[Category: Harlos K]]
-[[Category: Mcilhinney, R A.J.]]
+[[Category: Jones EY]]
-[[Category: Perestenko, P V.]]
+[[Category: McIlhinney RAJ]]
-[[Category: Seiradake, E.]]
+[[Category: Perestenko PV]]
-[[Category: Cell adhesion]]
+[[Category: Seiradake E]]
-[[Category: Synapse]]

3zyj: Difference between revisions

Latest revision as of 11:16, 20 December 2023

NetrinG1 in complex with NGL1

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

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