2lkr: Difference between revisions
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The | ==Yeast U2/U6 complex== | ||
<StructureSection load='2lkr' size='340' side='right'caption='[[2lkr]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2lkr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkr OCA], [https://pdbe.org/2lkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkr RCSB], [https://www.ebi.ac.uk/pdbsum/2lkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkr ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The U2/U6 snRNA complex is a conserved and essential component of the active spliceosome that interacts with the pre-mRNA substrate and essential protein splicing factors to promote splicing catalysis. Here we have elucidated the solution structure of a 111-nucleotide U2/U6 complex using an approach that integrates SAXS, NMR, and molecular modeling. The U2/U6 structure contains a three-helix junction that forms an extended "Y" shape. The U6 internal stem-loop (ISL) forms a continuous stack with U2/U6 Helices Ib, Ia, and III. The coaxial stacking of Helix Ib on the U6 ISL is a configuration that is similar to the Domain V structure in group II introns. Interestingly, essential features of the complex-including the U80 metal binding site, AGC triad, and pre-mRNA recognition sites-localize to one face of the molecule. This observation suggests that the U2/U6 structure is well-suited for orienting substrate and cofactors during splicing catalysis. | |||
Structure of the yeast U2/U6 snRNA complex.,Burke JE, Sashital DG, Zuo X, Wang YX, Butcher SE RNA. 2012 Feb 10. PMID:22328579<ref>PMID:22328579</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2lkr" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae]] | |||
[[Category: Burke JE]] | |||
[[Category: Butcher SE]] | |||
[[Category: Sashital DG]] | |||
[[Category: Wang Y]] | |||
[[Category: Zuo X]] | |||
