3t00: Difference between revisions

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[[Image:3t00.png|left|200px]]


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==Crystal Structure of Phosphonoacetate hydrolase from Sinorhizobium meliloti 1021 in complex with vanadate==
The line below this paragraph, containing "STRUCTURE_3t00", creates the "Structure Box" on the page.
<StructureSection load='3t00' size='340' side='right'caption='[[3t00]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3t00]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti_1021 Sinorhizobium meliloti 1021]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T00 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
{{STRUCTURE_3t00|  PDB=3t00  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t00 OCA], [https://pdbe.org/3t00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t00 RCSB], [https://www.ebi.ac.uk/pdbsum/3t00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t00 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q92UV8_RHIME Q92UV8_RHIME]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacteria have evolved pathways to metabolize phosphonates as a nutrient source for phosphorus. In Sinorhizobium meliloti 1021, 2-aminoethylphosphonate is catabolized to phosphonoacetate, which is converted to acetate and inorganic phosphate by phosphonoacetate hydrolase (PhnA). Here we present detailed biochemical and structural characterization of PhnA that provides insights into the mechanism of C-P bond cleavage. The 1.35 A resolution crystal structure reveals a catalytic core similar to those of alkaline phosphatases and nucleotide pyrophosphatases but with notable differences, such as a longer metal-metal distance. Detailed structure-guided analysis of active site residues and four additional cocrystal structures with phosphonoacetate substrate, acetate, phosphonoformate inhibitor, and a covalently bound transition state mimic provide insight into active site features that may facilitate cleavage of the C-P bond. These studies expand upon the array of reactions that can be catalyzed by enzymes of the alkaline phosphatase superfamily.


===Crystal Structure of Phosphonoacetate hydrolase from Sinorhizobium meliloti 1021 in complex with vanadate===
Structural and mechanistic insights into C-p bond hydrolysis by phosphonoacetate hydrolase.,Agarwal V, Borisova SA, Metcalf WW, van der Donk WA, Nair SK Chem Biol. 2011 Oct 28;18(10):1230-40. PMID:22035792<ref>PMID:22035792</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_22035792}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3t00" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 22035792 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22035792}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3t00]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T00 OCA].
[[Category: Sinorhizobium meliloti 1021]]
 
[[Category: Agarwal V]]
==Reference==
[[Category: Nair SK]]
<ref group="xtra">PMID:022035792</ref><references group="xtra"/>
[[Category: Nucleotide diphosphatase]]
[[Category: Sinorhizobium meliloti]]
[[Category: Agarwal, V.]]
[[Category: Nair, S K.]]
[[Category: Alkaline phosphatase superfamily]]
[[Category: Hydrolase]]
[[Category: Transition state mimic]]

Latest revision as of 10:17, 13 August 2026

Crystal Structure of Phosphonoacetate hydrolase from Sinorhizobium meliloti 1021 in complex with vanadate

3t00, resolution 1.80Å

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