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[[Image:1tez.gif|left|200px]]<br /><applet load="1tez" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1tez, resolution 1.80&Aring;" />
'''COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS'''<br />


==Overview==
==COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS==
DNA photolyases use light energy to repair DNA that comprises, ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine, dimers (CPDs). Here we report the crystal structure of a DNA photolyase, bound to duplex DNA that is bent by 50 degrees and comprises a synthetic, CPD lesion. This CPD lesion is flipped into the active site and split, there into two thymines by synchrotron radiation at 100 K. Although, photolyases catalyze blue light-driven CPD cleavage only above 200 K, this, structure apparently mimics a structural substate during light-driven DNA, repair in which back-flipping of the thymines into duplex DNA has not yet, taken place.
<StructureSection load='1tez' size='340' side='right'caption='[[1tez]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tez]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_6301 Synechococcus elongatus PCC 6301] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TEZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HDF:8-HYDROXY-10-(D-RIBO-2,3,4,5-TETRAHYDROXYPENTYL)-5-DEAZAISOALLOXAZINE'>HDF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TCP:5-METHYLTHYMIDINE'>TCP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tez OCA], [https://pdbe.org/1tez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tez RCSB], [https://www.ebi.ac.uk/pdbsum/1tez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tez ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHR_SYNP6 PHR_SYNP6] Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1tez_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tez ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place.


==About this Structure==
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair.,Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622<ref>PMID:15576622</ref>
1TEZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=HDF:'>HDF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] Known structural/functional Site: <scene name='pdbsite=ACT:Upon+Complexation+w.+The+Cpd-Damage+The+Active+Site+Appa+...'>ACT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15576622 15576622]
</div>
[[Category: Deoxyribodipyrimidine photo-lyase]]
<div class="pdbe-citations 1tez" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Synechococcus sp.]]
<references/>
[[Category: Carell, T.]]
__TOC__
[[Category: Essen, L.O.]]
</StructureSection>
[[Category: Klar, T.]]
[[Category: Large Structures]]
[[Category: Mees, A.]]
[[Category: Synechococcus elongatus PCC 6301]]
[[Category: FAD]]
[[Category: Synthetic construct]]
[[Category: HDF]]
[[Category: Carell T]]
[[Category: MG]]
[[Category: Essen L-O]]
[[Category: photolyase; dna repair; light-driven electron transfer]]
[[Category: Klar T]]
 
[[Category: Mees A]]
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