1tv2: Difference between revisions

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[[Image:1tv2.png|left|200px]]


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==Crystal structure of the hydroxylamine MtmB complex==
The line below this paragraph, containing "STRUCTURE_1tv2", creates the "Structure Box" on the page.
<StructureSection load='1tv2' size='340' side='right'caption='[[1tv2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1tv2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TV2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TV2 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BG5:5-HYDROXYAMINO-3-METHYL-PYRROLIDINE-2-CARBOXYLIC+ACID'>BG5</scene></td></tr>
{{STRUCTURE_1tv2|  PDB=1tv2  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tv2 OCA], [https://pdbe.org/1tv2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tv2 RCSB], [https://www.ebi.ac.uk/pdbsum/1tv2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tv2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MTMB1_METBA MTMB1_METBA] Catalyzes the transfer of the methyl group from monomethylamine to the corrinoid cofactor of MtmC (MtmC1 or MtmC2).<ref>PMID:9642198</ref> <ref>PMID:9195968</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.


===Crystal structure of the hydroxylamine MtmB complex===
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid.,Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK Chem Biol. 2004 Sep;11(9):1317-24. PMID:15380192<ref>PMID:15380192</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1tv2" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 15380192 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_15380192}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[1tv2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TV2 OCA].
 
==See Also==
*[[Amino Acids]]
 
==Reference==
<ref group="xtra">PMID:015380192</ref><references group="xtra"/>
[[Category: Methanosarcina barkeri]]
[[Category: Methanosarcina barkeri]]
[[Category: Chan, M K.]]
[[Category: Chan MK]]
[[Category: Ferguson, T K.]]
[[Category: Ferguson TK]]
[[Category: Gallucci, J.]]
[[Category: Gallucci J]]
[[Category: Hao, B.]]
[[Category: Hao B]]
[[Category: Kang, P T.]]
[[Category: Kang PT]]
[[Category: Krzycki, J A.]]
[[Category: Krzycki JA]]
[[Category: Soares, J A.]]
[[Category: Soares JA]]
[[Category: Zhao, G.]]
[[Category: Zhao G]]
[[Category: Tim barrel]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/1tv2"